@article{Hoi_Hue_Hang_Phuong_2019, title={Expression of <i>Oryza sativa</i> galactinol synthase gene in maize (Zea may L.)}, volume={41}, url={https://vjs.ac.vn/index.php/vjbio/article/view/13584}, DOI={10.15625/0866-7160/v41n2.13584}, abstractNote={&lt;p&gt;Galactinol synthase (GolS) is a key biological catalyst for the synthesis of the raffinose oligosaccharides (RFOs) which play important roles in abiotic stress adaptation of plants, especially drought tolerance. &lt;em&gt;GolS&lt;/em&gt; gene has been isolated on a variety of plants in order to create material resources for generating transgenic plants resistant to adverse environmental factors. In our previous research, we have isolated a &lt;em&gt;GolS&lt;/em&gt; gene from drought stress cDNA library of &lt;em&gt;Oryza sativa &lt;/em&gt;L. Moctuyen (named &lt;em&gt;OsGolS)&lt;/em&gt;. In this study, the expression vector pCAM-Rd/OsGolS carrying the isolated &lt;em&gt;OsGolS&lt;/em&gt; gene under the control of stress-inducible &lt;em&gt;Rd29A &lt;/em&gt;promoter was constructed and introduced into &lt;em&gt;Agrobacterium tumefaciens&lt;/em&gt; LBA4404, which was used for maize transformation.  PCR and Real-time PCR assay indicated that transgene was integrated in the genome of the regenerated &lt;em&gt;Zea mays&lt;/em&gt; plants. Reverse transcription-PCR showed that the &lt;em&gt;OsGolS&lt;/em&gt; was transcribed into mRNA in &lt;em&gt;Zea mays&lt;/em&gt; and was highly expressed. These results provide a basis for the study of the function of &lt;em&gt;OsGolS&lt;/em&gt; in drought responses and for the development of drought stress tolerant crops.&lt;/p&gt;&lt;p&gt;&lt;em&gt; &lt;/em&gt;&lt;/p&gt;}, number={2}, journal={Academia Journal of Biology}, author={Hoi, Pham Xuan and Hue, Huynh Thi Thu and Hang, Pham Thu and Phuong, Nguyen Duy}, year={2019}, month={Jun.} }