@article{Hong_Dat_Hoa_Cuong_Cuc_2019, title={Expression of a novel gene encoding protease inhibitor from metagenome of sponge in Vietnam}, volume={40}, url={https://vjs.ac.vn/index.php/vjbio/article/view/12759}, DOI={10.15625/2615-9023/v40n4.12759}, abstractNote={<p>Marine sponge is known as a “gold mine” of natural products from marine environment. Many novel bioactive compounds have been isolated from marine sponges and sponge-associated microorganisms such as antibiotics, anti-cancer compounds, protease inhibitors, etc. In this study, we selected a gene encoding protease inhibitor from metagenome of a sponge collected in Da Nang to express in <em>Escherichia coli </em>BL21(DE3)<em>. </em>The gene PI-DN9 encoding protease inhibitor (1.3 kb) was cut off cloning vector pUC57/PI-DN9 containing gene PI-DN9 and inserted into expression vector pET32a(+), the recombinant vector pET32a(+)/PI-DN9 then was transformed and expressed in the <em>E. coli</em> strain BL21(DE3). Results showed that recombinant protein (50 kDa) was expressed successfully at 25°C, 1 mM of IPTG in 5 hours. The recombinant protein was purified using Ni-NTA affinity chromatography column. Western blot assay and bioactive assay showed good activity of the purified protein.</p><p> </p>}, number={4}, journal={Academia Journal of Biology}, author={Hong, Tran Thi and Dat, Ton That Huu and Hoa, Nguyen Phuong and Cuong, Pham Viet and Cuc, Nguyen Thi Kim}, year={2019}, month={Dec.} }