@article{Hoai Thu_Viet_Minh_Bich Thuy_2018, title={The survey of expression levels of virulent genes in Salmonella strains isolated in retail meats in Hanoi}, volume={40}, url={https://vjs.ac.vn/index.php/vjbio/article/view/10633}, DOI={10.15625/0866-7160/v40n1.10633}, abstractNote={<p><em>Salmonella </em>is one of the major causes of food poisoning worldwide, especially in developing countries. The virulence of <em>Salmonella</em> is a complex process involving between their virulence factors and the host defenses. The purpose of this study was to detect virulent genes and to assess the level of expression of these genes in five <em>Salmonella</em> strains (including Enteritidis, Albany, Typhimurium, Hadar, and Derby), which were isolated from meat samples at the retail markets in Hanoi. As a result, <em>Salmonella</em> Enteritidis and <em>Salmonella</em> Typhimurium were 100% positive with seven virulent genes, e.g., <em>spiA</em>, <em>spvB</em>, <em>sitC</em>, <em>sifA</em>, <em>sipB</em>, <em>pagC</em>, and <em>invA </em>genes. <em>Salmonella </em>Hadar and <em>Salmonella</em> Derby positive with six genes, except <em>spvB</em>, whereas <em>Salmonella</em> Albany was positive with only the <em>pagC</em> gene. The comparision of the expression levels of these seven virulent genes and the <em>16S rRNA</em> control gene showed a significantly difference among <em>Salmonella</em> strains (<em>P</em><0.05). The result of gene expression of seven virulent genes indicate that <em>Salmonella</em> Hadar has the highest gene expression, followed by <em>Salmonella</em> Enteritidis, <em>Salmonella</em> Derby, <em>Salmonella</em> Typhimurium and finally <em>Salmonella</em> Albany. The results of molecular biology analysis will provide additional data on the expression of virulent genes in <em>Salmonella</em> strains isolated from retail meats in Hanoi.</p><p> </p>}, number={1}, journal={Academia Journal of Biology}, author={Hoai Thu, Nguyen Thi and Viet, Nguyen Thanh and Minh, Nghiem Ngoc and Bich Thuy, Vo Thi}, year={2018}, month={Jan.}, pages={62–68} }