Optimization of fermentation conditions for the expression of interleukin-3 in fusion with pelb in E. coli

Duong Thu Huong, Nguyen Thi Quy, Dang Thi Ngoc Ha, Le Thi Thu Hong, Truong Nam Hai
Author affiliations

Authors

  • Duong Thu Huong Institute of Biotechnology
  • Nguyen Thi Quy Institute of Biotechnology
  • Dang Thi Ngoc Ha Institute of Biotechnology
  • Le Thi Thu Hong Institute of Biotechnology
  • Truong Nam Hai Institute of Biotechnology

DOI:

https://doi.org/10.15625/0866-7160/v38n2.7126

Keywords:

Escherichia coli JM109, IL-3, PelB, protein expression.

Abstract

Interleukin-3 (IL-3) is a multifunctional cytokine which modulates the proliferation, differentiation and maturation of various types of hematopoietic cells. Gene coding for IL-3 linked with pelB signal were incorporated into pET22b(+) for  expression of il3 gene in E. coli BL21; but IL-3 was synthesized at very low levels and still in fusion with PelB. To facilitate purification process, finding the appropriate fermentation conditions plays a key role in order to enhance gene il-3 expression and cleavage of PelB. In this study, we have optimized the conditions for the expression of IL-3, which included E. coli host strain JM109, LB cultivation medium, induction temperature was 25oC; induction with 0.05 mM IPTG at OD600 = 1. The cell biomass increases at about 2.3 times after optimization. IL-3 protein was expressed in the form of inclusion body and the PelB signal was cleaved. This result is conducive for purification of large amount of IL-3 to determine characteristics of protein.

 

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Published

26-04-2016

How to Cite

Huong, D. T., Quy, N. T., Ngoc Ha, D. T., Thu Hong, L. T., & Hai, T. N. (2016). Optimization of fermentation conditions for the expression of interleukin-3 in fusion with pelb in E. coli. Academia Journal of Biology, 38(2), 250–256. https://doi.org/10.15625/0866-7160/v38n2.7126

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