Regeneration of Vigna radiata (L.) Wilczek via callus and ability for water stress tolerance of callus lines

Abstract

In this paper, we present results on plant regeneration in mungbean and drought tolerance at cullus level of eight mungbean cultivars (VN93-1; VN99-3; VC1973A; VC3902A; VC6148; VC6372; VC2768A, ĐX06). Vigna radiata (L.) Wilczek were regenerated via callus. The calli were formed from embryos on cullus induction medium (MS+10 mg/l 2,4D for VN93-1; VN99-3; VC1973A; VC3902A; VC6148; VC6372; VC2768A or MS+11 mg/l 2,4D for ĐX06). The formed calli were transfered onto shoots regeneration medium. MS medium added with 3 mg/l BAP was most suitable for shoot regeneration. Rate of shoot regeneration was from 77.3% to 98.6%. The MS medium added with 0.3 mg/l α-NAA gave the highest rate of  root and plant formation. 289 dehydration-tolerent callus lines and 715 plant lines have been generated.