Antioxidant, antibacterial and alpha amylase inhibitory activity of different fractions of Sonneratia apetala bark extract

Authors

  • Dao Van Tan HNUE
  • Mai Nhat Thuy

DOI:

https://doi.org/10.15625/0866-7160/v37n1se.6091

Keywords:

Sonneratia apetala, amylase inhibitor, antioxidant, antibacterial, Sep-pak plus cartridge

Abstract

Sonneratia apetala was imported into Vietnam in August 2003 and planted in XuanThuy National Park (Nam Dinh province). Samples of barks were collected and extracted with methanol and fractioned with ethyl acetate and 0.1% HCl in methanol using Sep-pak plus cartridges. Total polyphenol content of crude extract and the fractions was determined, and their antioxidant and antibacterial activity and Aspergillus niger α-amylase inhibitory activity was tested. Crude extract possessed the highest total polyphenol content (10973.2±2453.5 µg GAE/ml), followed by methanol-HCl fraction (124.6±10.4 µg GAE/ml) and ethyl acetate fraction (96.6±13.0 µg GAE/ml). Crude extract showed the highest antioxidant activity (IC50=3.4±0.3 µg/ml), equal to 0.86 folds of acid ascorbic activity using 1, 1-diphenyl-2- picrylhydrazyl (DPPH) scavenging assay, followed by methanol-HCl fraction (IC50=5.7±0.2 µg/ml) and ethyl acetate fraction (IC50=8.7±0.8 µg/ml). No antibacterial activity of ethyl acetate fraction was observed while the activity of crude extract against B. subtilis, S. aureus and that activity of methanol-HCl fraction against E. coli, B. subtilis were observed. The higher inhibition of Aspergillus niger α-amylase also was observed for the fractions of methanol-HCl (IC50=6.6±0.9 µg/ml) and ethyl acetate (IC50=16.9±1.0 µg/ml) compared to that of crude extract (IC50=300.4±66.0 µg/ml).

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Published

25-04-2015

How to Cite

Tan, D. V., & Thuy, M. N. (2015). Antioxidant, antibacterial and alpha amylase inhibitory activity of different fractions of Sonneratia apetala bark extract. Academia Journal of Biology, 37(1se), 54–60. https://doi.org/10.15625/0866-7160/v37n1se.6091

Issue

Section

Articles