Cloning Shrunken 2 (Sh2) gene from h14 maize cultivar and constructing transformation vector pcambia 1301-Ubi-Sh2

Abstract

The Shrunken 2 (Sh2) gene encodes the large subunit of endosperm ADP-glucose pyrophosphorylase – one of the enzymes that regulate the starch biosynthetic pathway. This gene had been transferred, successfully, to maize plant. The transgenic maize plants have higher starch content than that of wild type. In this article, we present the results on cloning the Sh2 gene by PCR from H14 maize cultivar and constructing transformation vector pCambia1301-Ubi-Sh2. The cloned Sh2 sequence had high similarity (99%) with sequence of Sh2 gene in Genbank with assesssion number NM_001127632.1. The sequence of Sh2 gene from H14 maize cultivar was submited to the GenBank with assession number JX462603. The gene was, then, successfully, inserted into expression vector pCambia1301 along with Ubiquitin promoter to construct a transformation vector pCambia 1301-Ubi-Sh2, that will be used for genetic engineering of starch in maize.