Effect of some cultivation conditions on biosurfactant production by Acinetobacter calcoaceticus H3 from Vietnam coastal zone
Keywords:Acinetobacter calcoaceticus, Emulsification index E24, biosurfactant, hydrocarbon, oil pollution treatment.
Having surface activity, emulsifiers, foaming agents, biosurfactants are widely used in many industries such as agriculture, chemistry, cosmetics, pharmaceutics, espencially in petroleum industry. Selecting marine biosurfactant-producing microoganisms and studying properties of biosurfactants are necessary to applying in oil pollution treatment. From marine sand samples taken from Vietnam coastal zone, a strain of biosurfactant-producing H3 was isolated. Strain H3 was identified as Acinetobacter calcoaceticus (id 99.9%) according to its morphological and 16S rDNA sequences. It grew and produced biosurfactant the most in medium with temperature 30oC, pH7, 0-1% NaCl (w/v) and DO as carbon source. In this condition, strain H3 produced crude biosurfactant of 15.73 g/l with the emulsification index E24 of 80%. Base on GC-MS analyses, the biosurfactant produced by Strain H3 consist of hydrophobic (-CH3) and hydrophilic (-COOH) groups in chemical structure C16H22O4 (1,2 benzendicarboxylic acide, bis 2- metyl propyl ester).