Genetic diversity of the tall-stilt mangrove (<em>Rhizophora apiculata</em> Blume) in Southern Central Coast of Vietnam using SSR markers

Nhung Hong Nguyen; Trong Dinh Nguyen; Giang Huong Thi Tran; Huy Quoc Nguyen; Ngoc Bich Pham; Ha Hoang Chu; Duy Vu Dinh; Phat Tien Do

doi:10.15625/2615-9023/21433

Author affiliations

Authors

Nhung Hong Nguyen
Trong Dinh Nguyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Giang Huong Thi Tran
Huy Quoc Nguyen Institute of Ecology and Works Protection, Vietnam Academy for Water Resources, Vietnam
Ngoc Bich Pham Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Ha Hoang Chu Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Duy Dinh Vu Join Vietnam–Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen, Ha Noi, Vietnam
Phat Tien Do Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam https://orcid.org/0000-0002-4227-7396

DOI:

https://doi.org/10.15625/2615-9023/21433

Keywords:

Genetic diversity, mangrove, Rhizophora apiculata, SSR markers, Vietnam

Abstract

Rhizophora apiculata is one of the important mangrove species for coastal communities and the global ecosystem. However, it has been threatening due to land clearing and over-exploitation, particularly in the Southern Central Coast of Vietnam. The SSR-PCR analysis indicated low levels of genetic diversity in R. apiculata populations distributed in the Southern Central regions of Vietnam. The observed number of alleles (N_A) was 1.933 indicating a low polymorphism for surveyed overall loci in tested populations. The observed heterozygosity (H_O) was also less than 0.5 indicating low heterozygosity at the species level. The interpopulation genetic diversity for each locus ranged from 0.000 to 0.188 demonstrating a moderate genetic differentiation among tested populations. Results of AMOVA analysis indicated that genetic variation almost occurred within the population (88%). Moreover, genetic distances of R. apiculata populations were low (drifted less than 0.05) indicating the gene pool exchange. At the overall population level, two different groups were observed and confirmed from UPGMA analysis and genetic distance matrix data. To gain a comprehensive understanding of the genetic diversity of R. apiculata in the Southern Central Coast of Vietnam, it would be necessary to utilize more molecular markers and investigate the matter further in other coastline regions. This would provide recommendations for mangrove tree breeding and restoring the mangrove forests in Vietnam as well as support for coping with the change of global climate.

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