High-frequency shoot multiplication of Laba banana (Musa sp.) cultured in vitro by using light, myo-inositol and adenin sulphate

Authors

  • Do Dang Giap Institute of Tropical Biology, VAST
  • Pham Ngoc Vinh Institute of Tropical Biology, VAST
  • Tran Trong Tuan Institute of Tropical Biology, VAST
  • Nguyen Thi Huyen Trang Institute of Tropical Biology, VAST
  • Pham Ngo Anh Thu Institute of Tropical Biology, VAST
  • Thai Xuan Du Institute of Tropical Biology, VAST

DOI:

https://doi.org/10.15625/0866-7160/v34n3se.1800

Keywords:

Musa sp., adenin sulphate, in vitro shoot, Laba banana, light intensity, myo-inositol

Abstract

The present paper describes the effect of light intensity, myo-inositol and adenin sulphate on in vitro shoot formation of Laba banana (Musa sp.). In vitro shoots were cultured on MS medium supplemented with 5 mg l-1 BA (6-benzylaminopurine) and different concentrations of myo-inositol (0, 100, 300, 500, 700 mg l-1) or adenin sulphate (0, 80, 100, 130, 160 mg l-1). After 4 weeks, the best multiple shoots were formed on MS medium with 5 mg l-1 BA + 100 mg l-1 adenin sulphate (6.9 shoots per explant) and on MS medium containing 5 mg l-1 BA + 100 mg l-1 myo-inositol (8.0 shoots per explant). Explants on MS medium with suitable concentration of BA were maintained under 12-hphotoperiod with different intensities of light: Diffuse light, 18.70 ± 1.00, 26.20 ± 1.00 and 42 ± 1.00 µmol m-2s-1. The best shoot formation rate (4.33 shoots per explant) was achieved on MS medium supplemented BA with a light intensity of 18.70 ± 1.00 µmol m-2s-1.

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Published

06-08-2012

How to Cite

Giap, D. D., Vinh, P. N., Tuan, T. T., Huyen Trang, N. T., Anh Thu, P. N., & Du, T. X. (2012). High-frequency shoot multiplication of Laba banana (Musa sp.) cultured in vitro by using light, myo-inositol and adenin sulphate. Academia Journal of Biology, 34(3se), 180–187. https://doi.org/10.15625/0866-7160/v34n3se.1800

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