High-frequency shoot multiplication of Laba banana (Musa sp.) cultured in vitro by using light, myo-inositol and adenin sulphate

Abstract

The present paper describes the effect of light intensity, myo-inositol and adenin sulphate on in vitro shoot formation of Laba banana (Musa sp.). In vitro shoots were cultured on MS medium supplemented with 5 mg l^-1 BA (6-benzylaminopurine) and different concentrations of myo-inositol (0, 100, 300, 500, 700 mg l^-1) or adenin sulphate (0, 80, 100, 130, 160 mg l^-1). After 4 weeks, the best multiple shoots were formed on MS medium with 5 mg l^-1 BA + 100 mg l^-1 adenin sulphate (6.9 shoots per explant) and on MS medium containing 5 mg l^-1 BA + 100 mg l^-1 myo-inositol (8.0 shoots per explant). Explants on MS medium with suitable concentration of BA were maintained under 12-hphotoperiod with different intensities of light: Diffuse light, 18.70 ± 1.00, 26.20 ± 1.00 and 42 ± 1.00 µmol m^-2s^-1. The best shoot formation rate (4.33 shoots per explant) was achieved on MS medium supplemented BA with a light intensity of 18.70 ± 1.00 µmol m^-2s^-1.