Creation in vitro materials and initial study of creating hairy root from Plumbago zeylanica L

Bui Dinh Thach; Nguyen Minh Hieu; Pham Thi Phuong Uyen; Ngo Ke Suong; Nguyen Huu Ho

doi:10.15625/0866-7160/v34n3se.1796

Authors

Bui Dinh Thach Institute of Tropical Biology, VAST
Nguyen Minh Hieu Institute of Tropical Biology, VAST
Pham Thi Phuong Uyen Institute of Tropical Biology, VAST
Ngo Ke Suong Institute of Tropical Biology, VAST
Nguyen Huu Ho Institute of Tropical Biology, VAST

DOI:

https://doi.org/10.15625/0866-7160/v34n3se.1796

Keywords:

Agrobacterium rhizogen, Plumbago zeylanica, hairy root, in vitro

Abstract

Plumbago zeylanica L. is a medicinal plant that contains high levels of the active ingredient plumbgin. With the goal of creating raw materials in vitro, 20-minute disinfection time, 10% solution is the optimum level to create templates in vitro (41.29%), MS medium supplemented with 1.5 mg/L BA and 0.1 mg L IBA create optimal shoots (4.67 shoots/sample) after 4 weeks of culture. In vitro shoots developed into the best tree in each MS supplemented with 0.1 mg / L IBA. Two strains of Agrobacterium rhizogen ATCC-15834 and ATCC-11325 used for transgenic stimulation ofhairy roots from in vitro leaves. The initial results have been selected for hairy root 5 lines.