Effects of some factors on friable callus induction and cell suspension culture of Panax Vietnamensis Ha et Grushv

Abstract

In the present study, the effects of 2,4-dichlorophenoxyacetic acid (2,4-D), a-naphthaleneacetic acid (NAA), mineral salt formulations, explant sources, cultural conditions on friable callus induction and cell suspension culture of Panax vietnamensis Ha et Grushv. were investigated. The results showed that the friable callus induction rate, fresh weight and dry weight were 1.6-fold higher when calli were cultured on media supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l NAA than those cultured on media with 2,4-D alone. The best medium for friable callus proliferation was Murashige and Skoog (MS) supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l NAA. Petiole explants cultured under 16/8 h light/dark photoperiod resulted in the best friable callus induction rate, fresh weight and dry weight (100%, 453 mg/explant, 23 mg/explant, respectively). After 8 weeks of culture, friable calli were transferred to MS liquid medium supplemented with 1.0 mg/l 2,4-D, 1.0 mg/l NAA and 30 g/l sucrose. The suspension cell cultures were maintained on a rotary shaker at 100 rpm. The maximum cell biomass of 23.67 mg/ml fresh weight was obtained after 14 days of culture.