A study on organogenesis from thin cell layer culture of Angelica acutiloba

Abstract

Angelica (Angelica acutiloba) plants originating in Japan were considered as of higher value than other varieties of the genus Angelica. Angelica’s roots have been used historically to treat health disorders and in many supplemental remedies in Asian traditional medicine. In this study, organogenesis from thin cell layer culture of japanese Angelica shoot tips and leaf blades was shown. Thin layers (1-1.5 mm thick) of shoot tips of Angelica were cultured on MS medium supplemented with NAA (0, 0.1, or 0.2 mg/l) and/or TDZ (0.1, 0.5 or 1 mg/l). The numbe of shoots was the largest (8.9 shoots/explant) when explants were cultured on the medium supplemented with 0.1 mg/l NAA and 1 mg/l TDZ. When Morel vitamins were replaced by Gamborg’s B5 vitamins together with the addition of 10% (v/v) coconut water and 40 mg/l adenine to the culture medium, the number of shoots per explants was remarkably increased after 6 weeks of culture.

In vitro Angelica leaves also proved a potential source for adventitious root production. On MS medium supplemented with kinetin (0 or 1 mg/l), NAA (4, 6, 8, 10 or 12 mg/l), IBA (4, 6, 8, 10 or 12 mg/l), the first open leaves from the shoot tip, when cultured in dark period, had a different response to the root formation. The root formation from japanese Angelica’s leaf culture significantly varied with the change of medium elements (minerals and vitamins). Percent of leaf explants having roots, number of roots per explant, root fresh and dry weights were the largest when leaf blades were cultured on the medium containing minerals and vitamins of Gamborg’s B5.