Identification and evaluation of enzymes gelatinase, amylase, and catalase produced by rice root endophytic bacteria isolated from Hai Duong with antimicrobial properties against \(\textit{Xanthomonas oryzea}\) pv. Oryzea

Nguyen Van Phuong; Nguyen Thuy Linh; Trinh Dinh Duy; Tran Le Nam Khanh; Do Doan Thu Giang; Ta Thi Thuy Linh; Mai Thi Phuong Nga; To Thi Mai Huong; Mai Duc Chung; Chu Hoang Ha; Le Tran Binh

doi:10.15625/2615-9023/15906

Author affiliations

Authors

Nguyen Van Phuong Graduate University of Science and Technology, VAST, Vietnam
Nguyen Thuy Linh University of Science and Technology of Hanoi, VAST, Vietnam
Trinh Dinh Duy University of Science and Technology of Hanoi, VAST, Vietnam
Tran Le Nam Khanh University of Science and Technology of Hanoi, VAST, Vietnam
Do Doan Thu Giang University of Science and Technology of Hanoi, VAST, Vietnam
Ta Thi Thuy Linh University of Science and Technology of Hanoi, VAST, Vietnam
Mai Thi Phuong Nga University of Science and Technology of Hanoi, VAST, Vietnam
To Thi Mai Huong University of Science and Technology of Hanoi, VAST, Vietnam
Mai Duc Chung Agricultural Genetics Institute, Hanoi, Vietnam
Chu Hoang Ha Institute of Biotechnology, VAST, Vietnam
Le Tran Binh Institute of Biotechnology, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/15906

Keywords:

Antagonistic activity, catalase, gelatin hydrolysis, endophytic bacteria, starch hydrolysis, rice, Xanthomonas oryzae pv.oryzae

Abstract

Endophytic bacteria (EB) possess different beneficial traits. Endophytic microbes are often functional in that they may carry nutrients from the soil into plants, modulate plant development, increase stress tolerance of plants, suppress virulence in pathogens, increase disease resistance in plants, and suppress development of competitor plant species. They may enhance plant development by carrying nutrients from the soil into plants and protect plants against phytopathogens by synthesizing extra-/intracellular proteolytic enzymes, as well as releasing antimicrobial metabolites and competing with pathogens for habitation and nutrients. In this study, we investigated the ability to produce exo/endo-enzymes such as gelatinase, amylase, and catalase, and antagonistic activity of 77 EB strains isolated from lowland rice roots grown in Doan Ket commune, Thanh Mien district, Hai Duong province, Vietnam. Out of 77 isolates, 76 (98.71%) showed the ability to liquefy gelatin after 7 days with different rates of hydrolysis. The test of starch hydrolysis revealed 58 (75.33%) isolates that were able to hydrolyze starch. Fifty-one out of 77 isolates (66.24%) were able to produce catalase. The antagonistic activity of rice root endophytic bacteria was determined against bacterial leaf blight disease-causing pathogen Xanthomonas oryzae pv. oryzae (Xoo), strains X19.2 and VX41. We found that three isolates (TP5, TP7, TP11) showed the ability to inhibit the growth of strain VX41 and twelve isolates (TP3, TP7, TP8, TP9, TP10, TP11, TP12, TP13, TP15, TP17, TP21, TP23) were able to inhibit the growth of strain X19.2. These results are served as a venue for further investigation in planta under the conditions of net house and field trials in order to confirm the potential strains for the development of bioinoculant toward controlling the disease caused by Xoo.

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