Identification and evaluation of enzymes gelatinase, amylase, and catalase produced by rice root endophytic bacteria isolated from Hai Duong with antimicrobial properties against \(\textit{Xanthomonas oryzea}\) pv. Oryzea

Nguyen Van Phuong, Nguyen Thuy Linh, Trinh Dinh Duy, Tran Le Nam Khanh, Do Doan Thu Giang, Ta Thi Thuy Linh, Mai Thi Phuong Nga, To Thi Mai Huong, Mai Duc Chung, Chu Hoang Ha, Le Tran Binh
Author affiliations

Authors

  • Nguyen Van Phuong Graduate University of Science and Technology, VAST, Vietnam
  • Nguyen Thuy Linh University of Science and Technology of Hanoi, VAST, Vietnam
  • Trinh Dinh Duy University of Science and Technology of Hanoi, VAST, Vietnam
  • Tran Le Nam Khanh University of Science and Technology of Hanoi, VAST, Vietnam
  • Do Doan Thu Giang University of Science and Technology of Hanoi, VAST, Vietnam
  • Ta Thi Thuy Linh University of Science and Technology of Hanoi, VAST, Vietnam
  • Mai Thi Phuong Nga University of Science and Technology of Hanoi, VAST, Vietnam
  • To Thi Mai Huong University of Science and Technology of Hanoi, VAST, Vietnam
  • Mai Duc Chung Agricultural Genetics Institute, Hanoi, Vietnam
  • Chu Hoang Ha Institute of Biotechnology, VAST, Vietnam
  • Le Tran Binh Institute of Biotechnology, VAST, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/15906

Keywords:

Antagonistic activity, catalase, gelatin hydrolysis, endophytic bacteria, starch hydrolysis, rice, Xanthomonas oryzae pv.oryzae

Abstract

Endophytic bacteria (EB) possess different beneficial traits. Endophytic microbes are often functional in that they may carry nutrients from the soil into plants, modulate plant development, increase stress tolerance of plants, suppress virulence in pathogens, increase disease resistance in plants, and suppress development of competitor plant species. They may enhance plant development by carrying nutrients from the soil into plants and protect plants against phytopathogens by synthesizing extra-/intracellular proteolytic enzymes, as well as releasing antimicrobial metabolites and competing with pathogens for habitation and nutrients. In this study, we investigated the ability to produce exo/endo-enzymes such as gelatinase, amylase, and catalase, and antagonistic activity of 77 EB strains isolated from lowland rice roots grown in Doan Ket commune, Thanh Mien district, Hai Duong province, Vietnam. Out of 77 isolates, 76 (98.71%) showed the ability to liquefy gelatin after 7 days with different rates of hydrolysis. The test of starch hydrolysis revealed 58 (75.33%) isolates that were able to hydrolyze starch. Fifty-one out of 77 isolates (66.24%) were able to produce catalase. The antagonistic activity of rice root endophytic bacteria was determined against bacterial leaf blight disease-causing pathogen Xanthomonas oryzae pv. oryzae (Xoo), strains X19.2 and VX41. We found that three isolates (TP5, TP7, TP11) showed the ability to inhibit the growth of strain VX41 and twelve isolates (TP3, TP7, TP8, TP9, TP10, TP11, TP12, TP13, TP15, TP17, TP21, TP23) were able to inhibit the growth of strain X19.2. These results are served as a venue for further investigation in planta under the conditions of net house and field trials in order to confirm the potential strains for the development of bioinoculant toward controlling the disease caused by Xoo.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Ahemad M., Kibret M., 2014. Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. J. King. Saud. Univ. - Sci., 26: 1–20.

Cappuccino J. G., Welsh C., 2017. Microbiology: A Laboratory Manual, 11th edition, Global edition. Pearson Education Limited, England, 560.

Choodamani M. S., Hariprasad P., Sateesh M. K., Umesha S., 2009. Involvement of catalase in bacterial blight disease development of rice caused by Xanthomonas oryzae pv. oryzae. Int. J. Pest. Manag., 55: 121–127.

El-shakh A. S. A., Kakar K. U., Wang X., Almoneafy A. A., Ojaghian M. R., Li B., Anjum S. I., Xie G., 2015. Controlling bacterial leaf blight of rice and enhancing the plant growth with endophytic and rhizobacterial Bacillus strains. Toxicol. Environ. Chem., 97: 766–785.

Fitri L., Ismail Y. S., Putriani P., Warzatullisna W., 2020. Application of Rice Root Endophytic Bacteria in Ciherang Variety Rice (Oryza sativa) Seeds. Biosaintifika, 12: 21–27.

Hameed A., Iqbal N., 2014. Chemo-priming with mannose, mannitol and H2O2 mitigate drought stress in wheat. Cereal. Res. Commun., 42: 450–462.

Inoue K., Suzuki T., Ikeda K., Jiang S., Hosogi N., Hyong G., Hida S., Yamada T., Park P., 2007. Extracellular matrix of Magnaporthe oryzae may have a role in host adhesion during fungal penetration and is digested by matrix metalloproteinases. J. Gen. Plant Pathol., 73: 388–398.

Jeung J. U., Heu S. G., Shin M. S., Vera Cruz C. M., Jean K. K., 2006. Dynamics of Xanthomonas oryzae pv. oryzae populations in Korea and their relationship to known bacterial blight resistance genes. Phytopathology, 96: 867–875.

Kennedy I. R., Choudhury A. T. M. A, Kecskés M. L. and Rose M. T., 2008. Efficient nutrient use in rice production in Vietnam achieved using inoculant biofertilisers. Proceedings of a project (SMCN/2002/073) workshop held in Hanoi, Vietnam, 12–13 October 2007. ACIAR Proceedings No. 130, pp. 49–59.

Leboffe M. J., Pierce B. E., 2010. Microbiology laboratory theory and application, 3rd ed. Morton Publishing Company, Englewood, CO.

Ly Ngoc Thanh Xuan, Tran Van Dung, Ngo Ngoc Hung, Cao Ngoc Diep, 2016a. Isolation and characterization of rice endophytic bacteria in acid sulphate soil of Mekong delta, Vietnam. World J Pharm Pharm Sci., 5: 301–317.

Ly Ngoc Thanh Xuan, Tran Van Dung, Ngo Ngoc Hung, Cao Ngoc Diep, 2016b. Isolation and characterization of rhizospheric bacteria in rice (Oryza sativa L.) cutivated on acid sulphates soils of the Mekong delta, Vietnam. World J Pharm Pharm Sci., 5: 343–358.

Mew T. W., Alvarez A. M., Leach J. E., Swings J., 1993. Focus on bacterial blight of rice. Plant Dis., 77: 5–12.

Moronta-Barrios F., Gionechetti F., Pallavicini A., Marys E., Venturi V., 2018. Bacterial microbiota of rice roots: 16S-based taxonomic profiling of endophytic and rhizospheric diversity, endophytes isolation and simplified endophytic community. Microorganisms, 6: 14.

Niño-Liu D. O., Ronald P. C., Bogdanove A. J., 2006. Xanthomonas oryzae pathovars: Model pathogens of a model crop. Mol. Plant. Pathol., 7: 303–324.

Nguyen Dac Khoa, Nguyen Dang Ngoc Giau, Tran Quoc Tuan, 2016. Effects of Serratia nematodiphila CT-78 on rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Biol. Control, 103: 1–10.

Nguyen Huynh Nha Uyen, Nguyen Thi Cam Van, Nguyen Dac Khoa, 2018. Comparison of the disease-reducing effects of the antagonistic Bacillus sp. Serratia nematodiphila against Xanthomonas oryzae pv. oryzae causing rice bacterial leaf blight. Can Tho Univ. J. Sci., 54(9B): 59–66. (in Vietnamese with English summary).

Nguyen Ngoc Lan, Vu Van Dung, Nguyen Thi Kim Lien, Nguyen Kim Thoa, Do Huu Nghi, Nguyen Huy Hoang, 2019. Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh provinces. Academia Journal of Biology, 41(4): 55–67.

Nguyen Van Phuong, Mai Thi Phuong Nga, To Thi Mai Huong, Mai Duc Chung, Chu Hoang Ha, Le Tran Binh, 2020. In vitro screesing for plant growth promoting traits and antifugal activity against Magnaporthe oryzea from submerged rice root endophytic bacteria. Proceedings of 2020 Vietnam National Conference on Biotechnology. Publishing House Hue University, pp. 568–574.

Olanrewaju O. S., Glick B. R., Babalola O. O., 2017. Mechanisms of action of plant growth promoting bacteria. World J. Microbiol. Biotechnol., 33: 1–16.

Phan Thi Cong, Tran Dang Dung, Tran Minh Hien, Nguyen Thanh Hien, Abu T. M. A. Choudhury, Mihaly L. Kecskes, Ivan R. Kennedy, 2009. Inoculant plant growth-promoting microorganisms enhance utilisation of urea-N and grain yield of paddy rice in southern Vietnam. Eur. J. Soil Biol., 45: 52–61.

Reinhold-Hurek B., Hurek T., 2011. Living inside plants: Bacterial endophytes. Curr. Opin. Plant Biol., 14: 435–443.

Sen S., Chandrasekhar C. N., 2014. Effect of PGPR on growth promotion of rice (Oryza sativa L .) under salt stress. Asian J. Plant Sci. Res., 4: 62–67.

Sofo A., Scopa A., Nuzzaci M., Vitti A., 2015. Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. Int. J. Mol. Sci., 16: 13561–13578.

Hoang Minh Tam , Cao Ngoc Diep, 2017. Isolation and characterization of endophytic bacteria isolated from the sugarcane cultivated on acrisols of Tay Ninh province,Vietnam. Int. J. Innov. Eng. Technol., 8: 222–236.

Trinh Thanh Trung, Dinh Thi Tuyet Van, Nguyen Phuong Lien, Dao Thi Luong, Duong Van Hop, 2017. Potential application on preparation for bio-fertilizer using Bacillus velezensis strains isolated from various regions in Vietnam. J. Biotechnol., 15:169–179 (in Vietnamese with English summary).

Van Thi Phuong Nhu, Cao Ngoc Diep, 2014. Isolation, charcaterization and phylogenetic analysis of endophytic bacteria in rice plant cultivated on soil of Phu Yen province, Vietnam. Am. J. Life Sci., 2: 117–127.

White J. F., Kingsley K. L., Zhang Q., Verma R., Obi N., Dvinskikh S., Elmore M. T., Verma S. K., Gond S. K., Kowalski K. P., 2019. Review: Endophytic microbes and their potential applications in crop management. Pest. Manag. Sci., 75: 2558–2565.

Zhao L. F., Xu Y. J., Lai X. H., 2018. Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties. Brazilian J, Microbiol., 49: 269–278.

Downloads

Published

30-06-2021

How to Cite

Phuong, N. V., Linh, N. T., Duy, T. D., Khanh, T. L. N., Giang, D. D. T., Linh, T. T. T., Nga, M. T. P., Huong, T. T. M., Chung, M. D., Ha, C. H., & Binh, L. T. (2021). Identification and evaluation of enzymes gelatinase, amylase, and catalase produced by rice root endophytic bacteria isolated from Hai Duong with antimicrobial properties against \(\textit{Xanthomonas oryzea}\) pv. Oryzea. Academia Journal of Biology, 43(2), 107–117. https://doi.org/10.15625/2615-9023/15906

Issue

Section

Articles