Evaluating the effects of simulated microgravity on mouse fibroblast

Hoang Nghia Son; Hoang Nguyen Quang Huy; Tran Thi Bich Tram; Ly Ngoc Cang; Ho Nguyen Quynh Chi; Truong Xuan Dai; Le Ngoc Phuong Thanh; Truong Thi Han; Nguyen Thai Minh Han; Le Thanh Long

doi:10.15625/2615-9023/v42n4.14919

Author affiliations

Authors

Hoang Nghia Son
Hoang Nguyen Quang Huy
Tran Thi Bich Tram
Ly Ngoc Cang
Ho Nguyen Quynh Chi
Truong Xuan Dai
Le Ngoc Phuong Thanh
Truong Thi Han
Nguyen Thai Minh Han
Le Thanh Long

DOI:

https://doi.org/10.15625/2615-9023/v42n4.14919

Keywords:

Cell cycle, mouse fibroblast, proliferation, simulated microgravity.

Abstract

The present study investigated how mouse fibroblasts changed under microgravity (SMG) conditions (< 10^-3 G) simulated by 3D clinostat. Results showed that SMG condition markedly reduced the proliferation of mouse fibroblasts, significantly reducing the nuclear area and intensity. Compared to the control group, the mouse fibroblasts ratio of the SMG group was higher in the G0/G1 phase but lower in the S phase and G2/M phase. The ratios of early and late apoptotic cells were also higher in the SMG group. The mouse fibroblasts under SMG conditions exhibited a reduction of β-Actin and α-Tubulin 3 expressions compared to the control group. These results suggested that the SMG condition diminished the proliferation and downregulated cytoskeletal protein expression of mouse fibroblasts.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Carvil P., Baptista R., Russomano T., 2013. The human body in a microgravity environment: Long term adaptations and countermeasures. Aviat. Focus, 4(1): 10–22.

Clément G., Slenzka K., 2006. Fundamentals of Space Biology: Research on Cells, Animals, and Plants in Space. Springer-Verlag New York Inc, New York, USA.

Crawford-Young S. J., 2006. Effects of microgravity on cell cytoskeleton and embryogenesis. Int. J. Dev. Biol., 50(2-3): 183–91.

Dubinin N. P., Vaulina E. N., 1976. The evolutionary role of gravity. Life Sci. Space Res., 14: 47–55.

Fletcher D., Mullins R., 2010. Cell mechanics and the cytoskeleton. Nature, 463:

–492.

Jiang C. J., Weeds A. G., Khan S., Hussey P. J. 1997. F-actin and G-actin binding are uncoupled by mutation of conserved tyrosine residues in maize actin depolymerizing factor (ZmADF). Proceedings of the National Academy of Sciences, 94(18): 9973–9978.

Kendall R. T., Feghali-Bostwick C. A., 2014. Fibroblasts in fibrosis: novel roles and mediators. Front. Pharmacol., 5: 123.

Kim Y. K., Park S. H., Lee J. H., Choi G. H., 2015. Design and Performance of an Automated Bioreactor for Cell Culture Experiments in a Microgravity Environment. J. Astron. Space Sci., 2(1): 81–89.

Lodish H., Kaiser C. A., Krieger M., Bretscher A., Ploegh H., Amon A., Scott M. P., 2012. Molecular Cell Biology. W.H. Freeman, Dallas, TX, USA chapter 4: 181–265.

McAnulty R. J., 2007. Fibroblasts and myofibroblasts: Their source, function and role in disease. Int. J. Biochem. Cell Biol., 39(4): 666–671.

Moes M. J. A., Bijvelt J. J., Boonstra J., 2007. Actin dynamics in mouse fibroblasts in microgravity. Microgravity Sci. Technol., 19: 180–183.

Morey-Holton E. R., 2003. The impact of gravity on life. In: Rothschild LJ, Lister AM (eds) Evolution on Planet Earth: The Impact of the Physical Environment, Academic Press, Cambridge, Massachusetts, USA pp. 143–146.

Ruden D. M., Bolnick A., Awonuga A., Abdulhasan M., Perez G., Puscheck E. E., Rappolee D. A., 2018. Effects of Gravity, Microgravity or Microgravity Simulation on Early Mammalian Development. Stem Cells Dev., 27(18): 1230–1236.

Sherr C. J., Beach D., Shapiro G. I., 2016. Targeting Cdk4 and Cdk6: From discovery to therapy. Cancer Discov., 6(4): 353–367.Wuest S. L., Richard S., Kopp S., Grimm D., Egli M., 2015. Simulated Microgravity: Critical Review on the Use of Random Positioning Machines for Mammalian Cell Culture. Biomed Res. Int., Article ID: 971474: 1–8.