Expression of Oryza sativa galactinol synthase gene in maize (Zea may L.)

Pham Xuan Hoi, Huynh Thi Thu Hue, Pham Thu Hang, Nguyen Duy Phuong
Author affiliations

Authors

  • Pham Xuan Hoi Viện Di truyền Nông Nghiệp - Viện Khoa học Nông nghiệp Việt Nam
  • Huynh Thi Thu Hue
  • Pham Thu Hang
  • Nguyen Duy Phuong

DOI:

https://doi.org/10.15625/0866-7160/v41n2.13584

Keywords:

Drought tolerance, maize transformation, Rd29A, OsGolS.

Abstract

Galactinol synthase (GolS) is a key biological catalyst for the synthesis of the raffinose oligosaccharides (RFOs) which play important roles in abiotic stress adaptation of plants, especially drought tolerance. GolS gene has been isolated on a variety of plants in order to create material resources for generating transgenic plants resistant to adverse environmental factors. In our previous research, we have isolated a GolS gene from drought stress cDNA library of Oryza sativa L. Moctuyen (named OsGolS). In this study, the expression vector pCAM-Rd/OsGolS carrying the isolated OsGolS gene under the control of stress-inducible Rd29A promoter was constructed and introduced into Agrobacterium tumefaciens LBA4404, which was used for maize transformation.  PCR and Real-time PCR assay indicated that transgene was integrated in the genome of the regenerated Zea mays plants. Reverse transcription-PCR showed that the OsGolS was transcribed into mRNA in Zea mays and was highly expressed. These results provide a basis for the study of the function of OsGolS in drought responses and for the development of drought stress tolerant crops.

 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

25-06-2019

How to Cite

Hoi, P. X., Hue, H. T. T., Hang, P. T., & Phuong, N. D. (2019). Expression of <i>Oryza sativa</i> galactinol synthase gene in maize (Zea may L.). Academia Journal of Biology, 41(2). https://doi.org/10.15625/0866-7160/v41n2.13584

Issue

Section

Articles

Most read articles by the same author(s)

<< < 1 2