Regulation of apoptosis of dendritic cells by il-10 and in association with stat-1 signaling molecule

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DOI:

https://doi.org/10.15625/0866-7160/v41n1.12607

Keywords:

Apoptosis, dendritic cell, IL-10 and STAT

Abstract

IL-10 is an anti-inflammatory cytokine, participating in induction of immune tolerance and cell apoptotic death. Dendritic cells (DCs) is the most professional antigen-presenting cells among innate immune cells to exert generation and maintenance of immunological memory mediated through activation of T and B lymphocytes. The STAT signalling pathway plays a regulatory role of maturation and differentiation of immune cells. In this study, DCs were treated with inflammatory cytokines including TNF-a, INFg, IL-2 and IL-10 and subsequently examined the phosphorylation of STAT-1 and STAT-3, TNF-α concetration in cell suspension and the proportion of Annexin V+ and caspase 3+ cells. Methods used for this investigation include western blotting, flow cytometry and ELISA. DCs were derived from mouse bone marrow cells and cultured with GM-CSF for 8 days. As a result, IL-10, but not other cytokines enhanced the number of Annexin V+cells and caspase 3 activity in DCs. More importantly, IL-10 also increased the phosphorylation of STAT-1 as well as the release of TNF-α into cell suspension. In conclusion, activation of STAT-1 might relate to the cell apoptotic death and TNF-α sectetion in IL-10-treated DCs.

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Author Biography

Nguyen Thi Xuan

Institute of Genome Research, Vietnam Academy of Science and Technology

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Published

28-03-2019

How to Cite

Thuy, N. T., & Xuan, N. T. (2019). Regulation of apoptosis of dendritic cells by il-10 and in association with stat-1 signaling molecule. Academia Journal of Biology, 41(1). https://doi.org/10.15625/0866-7160/v41n1.12607

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Articles