A constructive model of soil bio-cementing by using expanded glass granules
Keywords:soil cementing, biomineralization, bacteria, MICP
Soil bio-cementing is a promising technique for ground stabilization and/or production of unfired clay brick using bacteria. This study reported new findings using Sporosarcina pasteurii, a ureolytic baterium and a mixture of perfect particle package of expanded glass granules as a soil model. For bacteria growth, the nutrition solution was carefully prepared in the laboratory condition from different indigents such as casein, soymeal, NaCl, urea and CaCl2. Three mixtures containing large, medium and small granules were analyzed with regards to its compactness. As constated, round shape and high water absorption of glass granules helps us providing an ideal condition for biomineralization process. Precipitated products responsible for bonding glass beads were characterized with different techniques such as XRD, SEM. Calcium carbonate was regconized as the main generated product due to bacteria metabolism. Results of microscopic observation revealed the various position of bonding bridges between glass granules that placed side by side on petri dish and in the cylindrical mold. Obviously, the effect of curing time on the result of bonding materials was observed as a decisive factor for 7 and 21 days specimens. The model of soil particle packing from glass granules help us more easily to investigate the cementing ability of bacteria. From this study, we could consider future studies of soil biocementing with different strains of bacteria.
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