QUANTITATIVE DETERMINATION OF APIGENIN FROM APIUM GRAVEOLENS L. BY HPLC-PDA METHOD

Authors

  • Vi Nguyen Tuong Le Department of Pharmaceutical Biochemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
  • Dung Tien Le Department of Pharmaceutical Biochemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
  • Khoa Cuu Nguyen Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam. Department of Materials and Pharmaceutical Chemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Tuan Ngoc Nguyen
  • Hung Tong Quach Department of Pharmaceutical Biochemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam. Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
  • Phuong Thi Nguyen Inorganic Technology, Ho Chi Minh City University of Food Industry.
  • Hang Thi Le Dang Department of Materials and Pharmaceutical Chemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Chinh Van Nguyen Department of Materials and Pharmaceutical Chemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.
  • Quynh Tran Thuy Nguyen Department of Materials and Pharmaceutical Chemistry, Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam.

Abstract

In recent years, the study of the structure and biological activity of medicinal plants have a particularly important to search for anti-cancer medicine. Apium graveolens L. is a species that has been studied for its ability to anti-cancer, lower blood pressure, antioxidant ... In which, apigenin is the compound that is most interested in this species. Apigenin was isolated from the ethyl acetate extract and performed column chromatography with solvent system n-hexane: ethyl acetate (from 100% to 1: 1) obtained 7 fractions. Fraction 7 was applied on column chromatography with n-hexane: ethyl acetate (2:1). The qualitative and quantitative process of apigenin in Apium graveolens L. by HPLC - PDA method was investigated using reverse phase column VDSpher PUR 100 C18 (25 cm × 4,6 mm, 5 mm). with detection wavelength at 335 nm, the mobile phase is 0.1% acetic acid: acetonitrile (60:40) with isocratic elution, flow rate 1 mL/min; injection volume 20 µL; column temperature 25℃. Validation results showed that the process had high specificity, linearity, repeatability with RSD = 1,000%, accuracy recovery with 99,97%, LOD was 0,1 μg/mL and the LOQ was 0,3 μg/mL.


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References

. Lans CA. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. J Ethnobiol Ethnomed, 2006, 2(45).

. Yarnell E. Botanical medicines for the urinary tract. World J Urol, 2002, 20(5), 285-93.

. Khare CP. Indian medicinal plants: London. Springer Science Pub, 2008.

. Kooti W, Mansori E, Ghasemiboroon M Harizi M, Amirzargar A. Protective effects of celery (Apium graveolens) on testis and cauda epididymal spermatozoa in rat. Iran J Reprod Med, 2014, 12(5), 365-366.

. Mira PopoviÇ, Biljana KaurinoviÇ, Svetlana TriviÇ1, Neda Mimica-DukiÇ and Marija BursaÇ. Effect of Celery (Apium graveolens) Extracts on Some Biochemical Parameters of Oxidative Stress in Mice Treated with Carbon Tetrachloride. Phytotherapy research, Phytother. Res, 2006, 20, 531–537.

. Atta A. Anti-nociceptive and antiinflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol. 1998, 60(2), 117-24.

. Zidorn C, Johrer K, Ganzera M, Schubert B, Sigmund EM, Mader J, et al. Polyacetylenesfrom the apiaceae vegetables carrot, celery, fennel, parsley, and parsnip and their cytotoxic activities. J Agric Food chem, 2005, 53(7), 2518-23.

. Lans CA. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. J Ethnobiol Ethnomed, 2006, 2(45).

. Teng CM, Lee LG, Ko SN. Inhibition of platelet aggregation by apigenin from Apium graveolens. Asia Pac J Pharmacol, 1985, 3(85).

. Al-Howiriny T, Alsheikh A, Alqasoumi S, Al-Yahya M, ElTahir K, Rafatullah S. Gastric antiulcer, antisecretory and cytoprotective properties of celery in rats. Pharm Biol, 2010, 48(7), 786-793.

. Kooti W, Mansori E, Ghasemiboroon M Harizi M, Amirzargar A. Protective effects of celery (Apium graveolens) on testis and cauda epididymal spermatozoa in rat. Iran J Reprod Med, 2014, 12(5), 365-366.

. RK Parabathina; E Muralinath; PL Swamy; VVSN Harikrishna; KS Sree, J. Chem. Pharm. Res., 2011, 3, 816- 834.

. S Arora, S Vijay, D Kumar, J. Chem. Pharm. Res., 2011, 3, 145-150.

. VSP Chaturvedula; I Prakash, J. Chem. Pharm. Res., 2011.

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Published

2021-03-31

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Section

The 7th National Workshop on Research and Development of Natural Products