STRUCTURAL CHARACTERISTICS AND BIOLOGICAL ACTIVITY OF SULFATED POLYSACCHARIDE FROM RED ALGAE BETAPHYCUS GELATINUS

Authors

  • Van Thi Thanh Van NhaTrang Institute of Technology Research and Application, VAST
  • Hieu Mai Nhu Vo NhaTrang Institute of Technology Research and Application, VAST
  • Hang Thi Thuy Cao NhaTrang Institute of Technology Research and Application, VAST
  • Trinh Thi Hoai Phan NhaTrang Institute of Technology Research and Application, VAST
  • Duc Mai Tran NhaTrang Institute of Technology Research and Application, VAST
  • Thu Thi Minh Quach Institute of chemistry, VAST
  • Thuy Thi Thu Thanh Institute of chemistry, VAST

DOI:

https://doi.org/10.15625/2525-2518/58/6A/15598

Keywords:

Betaphycus gelatinus, biological activity, red algae, sulfated polysaccharide, structure

Abstract

In this paper, sulfated polysaccharides were extracted from red algae Betaphycus gelatinus species collected at Ninh Thuan sea by using several extraction methods. The structural characteristics of sulfated polysaccharides was investigated by IR and NMR methods. The results indicated that polysaccharide from Betaphycus gelatinus identified as hybrid carrageenan, mainly composed of β-carrageenan, κ-carrageenan and g-carrageenan. Biological activity evaluation showed that the sulfated polysaccharides possessed antioxidant activity with different levels.

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References

Craigie, J. S., and C Leigh. Carrageenans and agars. In: (J. A. Hellebust and J. S. Craigie, eds) Handbook of Phycological Methods, Physiological and Biochemical Melhods. Cambridge University Press Cambridge, (1978) 109—131.

Yermak, I. M., & Khotimchenko, Y. S. Chemical properties, biological activities and applications of carrageenan from red algae. In M. Fingerman, & R. Nagabhushanam (Eds.), Recent advances in marine biotechnology, 3 (2003) 207–255. New York: Science Publisher Inc.

Briones, A., Ambal, W., Estrella, R., Lanto, E., Sison, F., & Villanueva, M.. Anti-blood coagulant activity and hypocholesterolemic property of Philippine carrageenan. Philippine Journal of Science, 129 (2000) 85–91.

Necas, J., & Bartosikova, L.. Carrageenan: A review. Veterinary Medicine, 58 (2013)187–205.

Craigie, J (1990), ‘Cell wall’, In: KM Cole and RG Sheath (eds), Biology of the Red Algae , Cambridge, Cambridge University Press.

Vũ Ngọc Ban, Trần Nho Bốn, Phạm Hồng Hải, Trần Đình Toại.. Nghiên cứu ứng dụng Carrageenan từ rong đỏ Eucheuma gelatinae làm phụ gia chế biến thực phẩm, Tạp chí hóa học, 45(6A) (2007) 146-149.

Yaphe, W. and G. P. Arsenault.. Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides. Anal Biochem. 13 (1965) 143—148.

Dodgson KS. . Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 78 (1961) 312–319.

YapheW Colorimetric determination of 3,6-anhydrogalactose and galactose in marine algal polysaccharides. Anal Chem 10 (1960) 1327–1329.

G.-C. Yen, H.-Y. Chen, Antioxidant Activity of Various Tea Extracts in Relation to Their Antimutagenicity, J. Agric. Food Chem. 43 (1995) 27-32.

Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal. Biochem. 269 (1999) 337–341.

A.H. El-masry, H.H. Fahmy, S.H. Ali Abdelwahed, Synthesis and Antimicrobial Activity of Some New Benzimidazole Derivatives, Molecules 5 (2000) 1429-1438.

Stancioff, D. J. and N. F, Stanley.. Infrared and chemical studies on algal polysaccharides. Proc. Int. Seaweed Symp. 6 (1969) 595-609.

Pereira, L. (2006). Identification of phycocolloids by vibrational spectroscopy. In A. T. Critchley, M. Ohno, & D. B. Largo (Eds.), World seaweed resources – an authoritative reference system. ETI Information Services Ltd.

Fred van de Velde, Leonel Pereirac and Harry S. Rollema, The revised NMR chemical shift data of carrageenans, Carbohydrate Research 339 (2004) 2309–2313

E. Tojoa, J. Pradob, A simple 1H NMR method for the quantification of carrageenansin blends, Carbohydrate Polymers 53 (2003) 325–329.

F.van de Velde†‡S.H.Knutsen§1A.I.Usov∥H.S.Rollema†A.S.Cerezo, Review 1H and 13C high resolution NMR spectroscopy of carrageenans: application in research and industry, Trends in Food Science & Technology 13(3) (2002) 73-92.

Rafiquzzaman, S.M.; Ahmed, R.; Lee, J.M.; Noh, G.; Jo, G.A.; Kong, I.S. Improved methods for isolation of carrageenan from Hypnea musciformis and its antioxidant activity. J. Appl. Phycol, 28 (2016) 1265–1274

Youssouf, L.; Lallemand, L.; Giraud, P.; Soulé, F.; Bhaw-Luximon, A.; Meilhac, O.; D’Hellencourt, C.L.; Jhurry, D.; Couprie, J. Ultrasound-assisted extraction and structural characterization by NMR of alginates and carrageenans from seaweeds. Carbohydr. Polym. 166 (2017) 55–63.

Vázquez-Delfin E, Robledo D, Freile-Pelegrin Y Microwave assisted extraction of the carrageenan from Hypnea musciformis (Cystocloniaceae, Rohodophyta). J Appl Phycol, 26 (2014) 901–907.

Alves MGCF, Dore CMPG, Castro AJG, Nascimento MS, Cruz AKM,Soriano ME, Benevides MB, Leite EL Antioxidant, cytotoxic and hemolytic effects of sulfated galactans from edible red alga Hypnea musciformis. J Appl Phycol 24 (2012) 1217–1227.

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Published

2021-03-31

Issue

Section

The 7th National Workshop on Research and Development of Natural Products