QUANTITATIVE NUCLEAR MAGNETIC RESONANCE BASED ON PULCON METHODOLOGY: APPLICATION TO QUANTIFICATION OF SOME STANDARD MATERIAS AND NATURAL PRODUCTS
Quantitative nuclear magnetic resonance (qNMR) is not as widespread as structural NMR, but has recently received increasing attention from the analytical community due to its advantages such as nondestructive sampling, fast analysis, no need to prepare a calibration curve, the reference standard is not identical to the analyte so that one reference material can be used to find the purity of any compound.
Traditional qNMR uses internal standard method, where sample and the reference standard are mixed together in the NMR tube so both sample and reference standard integrity is an issue after analysis. In PULCON (Pulse Length–based Concentration), a relatively new qNMR method using external standards, analytical samples and standards are always separated during qNMR experiment, not mixed together, thus integrity is not an issue for both sample and reference standard.
Currently, both internal standard and external standard qNMR are not widely disseminated in Vietnam. In this report, the ERETIC qNMR procedure was introduced on the 1H qNMR experiments of two high purity substances and three natural products, extracted in Vietnam. The results obtained with the PULCON qNMR method were compared to the reference data from manufactories or those obtained by traditional internal standard qNMR and were found to be in reasonable agreement. Thus, PULCON qNMR can be of wide utility for routine analysis, especially for the expensive and new compounds.
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