EXTRACTION OF TRITERPENOIDS FROM THE VIETNAMESE RED GANODERMA LUCIDUM BY ULTRASOUND-ASSISTED EXTRACTION METHOD AND ANTI-OXIDANT ACTIVITY OF EXTRACT
Keywords:Ganoderma lucidum, ultrasound – assisted extraction, response surface methodology, triterpenoids
An ecofriendly ultrasound – assisted extraction (UAE) method was developed for the triterpenoids extraction from Vietnamese red Ganoderma lucidum (G. lucidum). The appropriate kind of solvent for extraction procedure was selected as ethanol based on the yield of triterpenoids obtained, followed by the investigation of four single factors including solvent-to-material ratio, extraction temperature, extraction time, and ultrasonic power. According to the result of single factor experiments, optimal extraction conditions were determined with solvent-to-material ratio of 30:1 mL/g, extraction temperature of 55 , extraction time of 60 min, and ultrasonic power of 480 W. Under these conditions, UAE produced a highest triterpenoids yield of 9.7976 0.334 mg/g by using ultraviolet-visible spectroscopy (UV-Vis) analysis. Extract obtained from UAE method showed better antioxidant activity compared to that of ascorbic acid with the half-maximal inhibitory concentration values were of 32.685 and 6.902 µg/mL, respectively. As a result, triterpenoids extracted from G. lucidum could be considered as a promising antioxidant agent.
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