EXTRACTION AND BIOACTIVITY EVALUATION OF THE EXTRACTS FROM CLEISTOCALYX OPERCULATUS L. LEAVES
Keywords:Cleistocalyx Operculatus L., extraction, anti-inflammatory, anti-bacterial, antioxidant
AbstractCleistocalyx Operculatus L. was known as a natural resource of bioactivity compounds with high potential of anti-inflammatory, anti-bacterial, and antioxidant. In this study, two conventional extraction methods (maceration and liquid-solid extraction) at different extraction conditions (solvent concentration, extraction temperature and time, liquid-to-solid ratio, and number of extractions) were conducted. The extracts were analyzed for total phenolic content, anti-inflammatory activity, anti-bacterial, and antioxidant activity. Three parameters (liquid-solid ratio, extraction temperature, and time) were the important factors that affected on the extraction yield and antioxidant activity. The results showed that the highest values of the extraction yield and antioxidant activity were obtained at liquid-solid ratio of 5:1 (v/w – mL/g), extraction temperature and time of 50°C and 50 min, respectively. The antioxidant activity of the extract was confirmed by IC50 values (29.94 ± 2.34 μg/mL). Moreover, Cleistocalyx Operculatus L. extract shown an average anti-inflammatory activity (381.14 ± 5.48 μg/mL), while it did not exhibit anti-bacterial activity at 1,000 mg/L.
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