• Nguyen Thi Ngoc Tuyet Refinery and Petrochemicals Technology Research Center (RPTC), Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Viet Nam https://orcid.org/0000-0003-1129-3577
  • Nguyen Minh Anh Refinery and Petrochemicals Technology Research Center (RPTC), Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Viet Nam
  • Le Thi Kim Phung Refinery and Petrochemicals Technology Research Center (RPTC), Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Viet Nam




Cleistocalyx Operculatus L., extraction, anti-inflammatory, anti-bacterial, antioxidant


Cleistocalyx Operculatus L. was known as a natural resource of bioactivity compounds with high potential of anti-inflammatory, anti-bacterial, and antioxidant. In this study, two conventional extraction methods (maceration and liquid-solid extraction) at different extraction conditions (solvent concentration, extraction temperature and time, liquid-to-solid ratio, and number of extractions) were conducted. The extracts were analyzed for total phenolic content, anti-inflammatory activity, anti-bacterial, and antioxidant activity. Three parameters (liquid-solid ratio, extraction temperature, and time) were the important factors that affected on the extraction yield and antioxidant activity. The results showed that the highest values of the extraction yield and antioxidant activity were obtained at liquid-solid ratio of 5:1 (v/w – mL/g), extraction temperature and time of 50°C and 50 min, respectively. The antioxidant activity of the extract was confirmed by IC50 values (29.94 ± 2.34 μg/mL). Moreover, Cleistocalyx Operculatus L. extract shown an average anti-inflammatory activity (381.14 ± 5.48 μg/mL), while it did not exhibit anti-bacterial activity at 1,000 mg/L.


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Subhadrabandhu S. - Under-Utilized Tropical Fruits of Thailand, Thailand, 2001.

Nguyen P. T. M., Schultze N., Boger C., Alresley Z., Bolhuis A., and Lindequist U. - Anticaries and antimicrobial activities of methanolic extract from leaves of Cleistocalyx operculatus L., Asian Pac. J. Trop. Biomed. 7 (1) (2017) 43-48. https://doi.org/10.1016/j.apjtb.2016.11.009.

Tran G. B., Le N. T. T. and S. M. Dam - Potential use of essential oil isolated from Cleistocalyx operculatus leaves as a topical dermatological agent for treatment of burn wound, Dermatol. Res. Pract. 2018 (2018) 2730169. https://doi.org/10.1155/2018/2730169.

Min B. S., Thu C. V., Dat N. T., Dang N. H., Jang H. S. and Hung T. M. - Antioxidative flavonoids from Cleistocalyx operculatus buds, Chem. Pharm. Bull. 56 (12) (2008) 1725-1728. https://doi.org/10.1248/cpb.56.1725.

Dung N. T., Kim J. M. and Kang S. C. - Chemical composition, antimicrobial and antioxidant activities of the essential oil and the ethanol extract of Cleistocalyx operculatus (Roxb.) Merr and Perry buds, Food Chem. Toxicol. 46 (12) (2008) 3632-3639. https://doi.org/10.1016/j.fct.2008.09.013.

Dung N. T., Bajpai V. K., Yoon J. I. and Kang S. C. - Anti-inflammatory effects of essential oil isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr and Perry, Food Chem. Toxicol. an Int. J. Publ. Br. Ind. Biol. Res. Assoc. 47 (2) (2009) 449-453. https://doi.org/10.1016/j.fct.2008.11.033.

Dosoky N. S., Pokharel S. K. and Setzer W. N. - Leaf essential oil composition, antimicrobial and cytotoxic activities of Cleistocalyx operculatus from Hetauda, Nepal, Am. J. Essent. Oils Nat. Prod. 2 (5) (2015) 34–37.

Le P. T. K., Vo H. P. H., Vu Q. T. H., Nguyen T. T. N. and Le K. A. - Grape processing by-product as a source of nutraceutical components, Chem. Eng. Trans. 63 (2018) 205-210. https://doi.org/10.3303/CET1863035.

Le P. T. K., Vu Q. T. H., Nguyen Q. T. V., Tran K. A. and Le K. A. - Extraction and evaluation the biological activities of oil from spent coffee grounds, Chem. Eng. Trans. 56 (2017) 1729–1734. https://doi.org/10.3303/CET1756289.

Miguel M.G. - Antioxidant activity of medicinal and aromatic plants, Flavour Fragr. J. 25 (2009) 291-312. https://doi.org/10.1002/ffj.

Mai T. T., Thu N. N., Tien P. G. and Chuyen N. V. - Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents, J. Nutr. Sci. Vitaminol. (Tokyo). 53 (3) (2007) 267-276. https://doi.org/10.3177/jnsv.53.267.

Cerretani L. and Bendini A. - Chapter 67: Rapid Assays to Evaluate the Antioxidant Capacity of Phenols in Virgin Olive Oil, Eds. San Diego: Academic Press, 2010, pp. 625-635.

Zhou K. and Yu L. - Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado, LWT - Food Sci. Technol. 39 (10) (2006) 1155-1162. https://doi.org/10.1016/j.lwt.2005.07.015.

Gawron-Gzella A., Królikowska A. and Pietrzak M. - Antioxidant activity of teas obtained from leaves of Camellia sinensis (L.) Kuntze in course of various production processes available on Polish market, Herba Pol. 64 (2) (2018) 60-67. https://doi.org/10.2478/hepo-2018-0007.

Verma M., Rai G. K. and Kaur D. - Effect of extraction solvents on phenolic content and antioxidant activities of Indian gooseberry and guava, Int. Food Res. J. 25 (2) (2018) 762-768.

Londhe J. S., Devasagayam T. P. A., Foo L. Y. and Ghaskadbi S. S. - Antioxidant activity of some polyphenol constituents of the medicinal plant Phyllanthus amarus Linn, Redox Rep. 13 (5) (2008) 199-207. https://doi.org/10.1179/135100008X308984.

Jadhav H. R. and Bhutani K. K. - Antioxidant properties of Indian medicinal plants, Phyther. Res. 16 (8) (2002) 771-773. https://doi.org/10.1002/ptr.1063.

Hasan A. E. Z., Nashrianto H., Juhaeni R. N. and Artika I. M. - Optimization of conditions for flavonoids extraction from Mangosteen (Garcinia mangostana L.), Der Pharm. Lett. 8 (18) (2016) 114-120.

Koffi E., Sea T., Dodehe Y. and Soro S. - Effect of solvent type on extraction of polyphenols from twenty three Ivorian plants., J. Anim. Plant Sci. 5 (3) (2010) 550-558.

Sun C., Wu Z., Wang Z. and Zhang H. - Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of Beijing propolis extracts, Evidence-based Complement. Altern. Med. (2015) 595393. https://doi.org/10.1155/2015/595393.

Waszkowiak K. and Gliszczyńska-Świgło A. - Binary ethanol–water solvents affect phenolic profile and antioxidant capacity of flaxseed extracts, Eur. Food Res. Technol. 242 (5) (2016) 777-786. https://doi.org/10.1007/s00217-015-2585-9.

He G. Q., Xiong H. P., Chen Q. H., Ruan H., Wang Z. Y. and Traoré L. - Optimization of conditions for supercritical fluid extraction of flavonoids from hops (Humulus lupulus L.), J. Zhejiang Univ. Sci. 6B (10) (2005) 999-1004. https://doi.org/10.1631/jzus.2005.B0999.

Larrauri J. A., Rupérez P. and Saura-Calixto F. - Effect of drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels, J. Agric. Food Chem. 45 (4) (1997) 1390-1393. https://doi.org/10.1021/jf960282f.

Akowuah G. A., Mariam A. and Chin J. H. - The effect of extraction temperature on total phenols and antioxidant activity of Gynura procumbens leaf, Pharmacogn. Mag. 4 (17) (2009) 81-85.

Nicola E. D., Owen J. C., John B. G., Rosemary F. W., Kevin A. M., Foo L. Y. and Nigel B. P. - Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures, Food Chem. 101 (4) (2007) 1417-1424. https://doi.org/10.1016/j.foodchem.2006.03.050.

Yaqin X., Rui Z. and Hong F. - Studies on the optimal process to extract flavonoids from red raspberry fruits, Nat. Sci. 3 (2) (2005) 1689-1699. https://doi.org/10.1017/CBO9781107415324.004.

Sibel Y. - Modeling of solid-liquid extraction of total phenolics from Capsicum, J. Turkish Chem. Soc. Sect. B Chem. Eng. 1 (1) (2017) 43-60.

Cacace J. E. and Mazza G. - Mass transfer process during extraction of phenolic compounds from milled berries, J. Food Eng. 59 (4) (2003) 379-389. https://doi.org/10.1016/S0260-8774(02)00497-1.

Zhang S., Bi H. and Liu C. - Extraction of bio-active components from Rhodiola sachalinensis under ultra-high hydrostatic pressure, Sep. Purif. Technol. 57 (2) (2007) 277-282. https://doi.org/10.1016/j.seppur.2007.04.022.

Herodež Š. S., Hadolin M., Škerget M. and Knez Ž. - Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves, Food Chem. 80 (2) (2003) 275-282. https://doi.org/10.1016/S0308-8146(02)00382-5.

Al-Farsi M. A. and Lee C. Y. - Optimization of phenolics and dietary fibre extraction from date seeds, Food Chem. 108 (3) (2008) 977-985. https://doi.org/10.1016/j.foodchem.2007.12.009.

Wu S. D., Jiang X. Y., Chen Q. Y. and Chen X. Q. - Comparison of techniques for the extraction of the hypotensive drugs geniposidic acid and geniposide from Eucommia ulmoides, J. Iran. Chem. Soc. 4 (2) (2007) 205-214. https://doi.org/10.1007/BF03245968.






The 7th National Workshop on Research and Development of Natural Products