INVESTIGATION OF ANTI-HELICOBACTER PYLORI ACTIVITY AND CHEMICAL CONSTITUENTS OF LUDWIGIA HYSSOPIFOLIA
Keywords:antimicrobial, disk diffusion, Helicobacter pylori, Ludwigia hyssopifolia, sterol
Helicobacter pylori (H. pylori) is considered as the main cause of peptic ulcer and gastric carcinoma. Due to the growing trend of antimicrobial resistance, the control of H. pylori infections need to be recommended. Recently, screening for natural sources with potential antimicrobial activity is a strong demand. Ludwigia hyssopifolia was traditionally used so far for treatment of H. pylori infection in Mekong Delta region of Vietnam. In this study, the five extracts of Ludwigia hyssopifolia including n-hexane, chloroform, ethyl acetate, methanol and ethanol ones were screened for anti H. pylori activity by disk diffusion method. The preliminary results showed that all these five extracts displayed a moderate activity in which the methanol one exhibited a stronger activity with the inhibition zone (d = 17 mm) at the concentration of 0.5 mg/disk. Phytochemical study on the bioactive methanol extract led to the isolation of four known compounds as β-sitosterol (1), oleanolic acid (2), 3-O-β-D-glucopyranosyl-β-sitosterol (3), and ellagic acid (4). Their structures were elucidated by spectroscopic methods including 1D and 2D-NMR and in comparison with literature data.
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