ANTIFUNGAL ACTIVITY OF COUMARIN AND TRITERPENOID TARAXASTERYL ACETATE FROM THE N-HEXANE SOLUBLE FRACTION OF THE AERIAL PART OF EUPATORIUM FORTUNE AGAINST SCLEROTIUM ROLFSSI AND RHIZOCTONIA SOLANI

Authors

  • Trang Thi Thu Bui
  • Quynh Trang Thi Pham
  • Trung Hieu Pham
  • Thanh Huong Nguyen
  • Thoa Thi Vu
  • Duyen Thi Nguyen
  • Quang Le Dang Vietnam Institute of Industrial Chemistry https://orcid.org/0000-0001-7203-8081

DOI:

https://doi.org/10.15625/2525-2518/58/6A/15414

Keywords:

Eupatorium fortune, coumarin, antifungal activity, botanical fungicides

Abstract

Abstract-HCTN7. Phytopathogenic fungi Sclerotium rolfssi and Rhizoctonia solani in the form of sclerotia can survive in the soil for a long time and cause serious disease severity by attacking the roots and lower stems of plants. There are challenges in the control of the plant fungal diseases by using chemical fungicides. During screening antifungal-plant, we found that the n-hexane soluble fraction of Eupatorium fortune highly inhibited the mycelia growth of both phytopathogenic fungi. The bioassay-guided fractionation of the hexane soluble fraction led to the isolation of antifungal compounds coumarin (1) and taraxasteryl acetate (2). The other compounds scopoletin (3), and scololin (4) were isolated from the ethyl acetate soluble fraction of E. fortune. The chemical structures of 1-4 were elucidated by 1D- and 2D-NMR spectroscopy and ESI-MS methods. The isolated compounds were evaluated their antifungal activity against S. rolfssi and R. solani by in vitro food poisoned technique bioassay. The mycelial growth of R. solani was markedly inhibited by 89.7% and 85.8% when exposed to 1 after 1 day and 2 days treatments at 250 ppm, respectively. Compound 2 displayed the most antifungal activity; it inhibited the mycelia growth of S. rolfssi by 98.7 and 89.4 % at 250 and 100 ppm 1 day after treatment, respectively.  This study indicated that the aerial part of E. fortune possesses potent in vitro antifungal activity against S. rolfssi and R. solani and contains bioactive compounds that could be used as active ingredients for developing botanical fungicides against plant diseases.  

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Author Biography

Quang Le Dang, Vietnam Institute of Industrial Chemistry

Director/Senior Researcher, R&D Center of Bioactive Compounds

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Published

2021-03-31

Issue

Section

The 7th National Workshop on Research and Development of Natural Products