• Dat Quoc Lai Faculty of Chemical Engineering, Ho Chi Minh City University of Technology
  • An Thanh Ngo Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Phung Thi Kim Le Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Dung Hoang Nguyen Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)
  • Long Quang Nguyen Faculty of Chemical Engineering, Ho Chi Minh City University of Technology (HCMUT)



rice bran, gamma-amino butyric acid, nanofiltration, purification


This research focused on technical assessment of GABA production from rice bran through fermentation by Lactobacillus brevis. Influence of operating pressure on separation of GABA by nanofiltration membrane was investigated and 4 bar was suitable for the nanofiltration process. The purification of GABA by nanofiltration with constant feed volume was carried out and purity of GABA reached 4.8 folds, compared to feed, at 5 volumes of added water. At 40 of concentration factor in concentration of GABA solution by nanofiltration with full recycle of retentate, content of GABA reached 49.8 g/L. The production of GABA from defatted rice bran at pilot scale was carried out at 1,000 L/batch (equal to 200 kg of rice bran) of fermentation. Estimation of mass balance showed that, with 200 kg of defatted rice bran, 7.0 kg of GABA powder was obtained. Results indicated that, it is potential to produce GABA from rice bran through the fermentation by Lactobacillus brevis.


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AOAC Official Methods of Analysis, 15th Ed.( 1990) Methods 32.034 - 32.039.

Aoki H., Uda I., Tagami K., Furuya Y., Endo Y. and Fujimoto K. - The Production of a New Tempeh-like Fermented Soybean Containing a High Level of g -Aminobutyric Acid by Anaerobic Incubation with Rhizopus, Bioscience, Biotechnology, and Biochemistry 67 (2003) 1018–1023.

Bjork M.J., Moeller F.G., Kramer L.G., Kram M., Suris A., Rush A.J. and Petty F. - Plasma GABA levels correlate with aggressiveness in relatives of patients with unipolar depressive disorder, Psychiatry Research 101 (2) (2001) 131-136.

Hayakawa K., Kimura M. and Kamata K. - Mechanism underlying γ-aminobutyric acid-induced antihypertensive effect in spontaneously hypertensive rats, European Journal of Pharmacology 438 (1–2) (2002) 107-113.

Hussain A. A., Abashar M. E. E. and Al-Mutaz I. S. - Influence of ion size on the prediction of nanofiltration membrane systems, Desalination 214 (2007) 150 -166

Ichimura T., Yamanaka A., Ichiba T., Toyokawa T., Kamada Y., Tamamura T. and Maruyama S. - Antihypertensive Effect of an Extract of Passiflora edulis Rind in Spontaneously Hypertensive Rats, Bioscience, Biotechnology, and Biochemistry 70 (3) (2006) 718-721.

Lai Q. D., Tran T. K. N. - Nguyen T. X. N. - Gamma-amino butyric acid (GABA) synthesis of Lactobacillus in fermentation of defatted rice bran extract, AIP Conference Proceedings 1878 (2017) 020045

Luh S. B. - Rice: Production and Utilization. Second Edition. AVI Publishing Corp, 1991, pp. 925.

Moo-Chang K., Seo M.J., Cheigh C.I., Pyun Y.R., Cho S.C. and Park H. - Enhanced production of γ-aminobutyric acid using rice bran extracts by Lactobacillus sakei B2-16, Journal of Microbiology and Biotechnology 20 (4) (2010) 763–766.

Pontalier P., Ismail A. and Ghoul M. - Mechanism for the selective rejection of solutes in nanofiltration membranes, Separation and Purification Technology 12 (1997) 175 – 181

Saikusa T., Horino T. and Mori Y. - Accumulation of γ-Aminobutyric Acid (Gaba) in the Rice Germ during Water Soaking, Bioscience, Biotechnology, and Biochemistry 58 (12) (1994) 2291-2292.

Szymczyk A., Labbez C., Fievet P., Vidonne A., Foissy A. and Pagetti J. - Contribution of convection, diffusion and migration to electrolyte transport through nanofiltration membranes, Advances Colloid and interface science 103 (2003) 77-94.

Tsushida T. and Murai T. - Conversion of Glutamic Acid to γ-Aminobutyric Acid in Tea Leaves under Anaerobic Conditions, Agricultural and Biological Chemistry 51 (11) (1987) 2865-2871.

Vaiva G., Thomas P., Ducrocq F., Fontaine M., Boss V., Devosc P., Rascle C., Cottencin O., Brunet A., Laffargue P. and Goudeman M. (2004): Low posttrauma GABA plasma levels as a predictive factor in the development of acute posttraumatic stress disorder. Biological Psychiatry 55 (3) (2004) 250-254.

Vi N. T., Le T. K. P. and Lai Q. D. - Purification of Gamma-Amino Butyric Acid (GABA) from Fermentation of Defatted Rice Bran Extract by Using Ion Exchange Resin, AIP Conference Proceedings 1878 (2017) 020007

Vincze I. and Vatai G. - Application of nanofiltration for coffee extract concentration, Desalination 162 (2004) 287 – 294.

Van der Horst H. C., Timmer J. M. K.; Robbertsen T. and Leenders J. - Use of nanofiltration for concentration and demineralization in the dairy industry: Model for mass transport, Journal of Membrane Science 104 (1995) 205 218.

Warczok J., Ferrando M., López F. and Guell C. - Concentration of apple and pear juices by nanofiltration at low pressure, Journal of Food Engineering 63 (2004) 63 – 70.

Watanabe M., Maemura K., Kanbara K., Tamayama T. and Hayasaki H. - GABA and GABA receptors in the central nervous system and other organs, International Review of Cytolog 213 (2002) 1–47.

Wijmans J. G., Nakao S., and Smolders C. A. (1984). Flux limitation in ultrafiltration: Osmotic pressure model and gel layer model, Journal of Membrane Science 20 (1984) 115 – 124.

Zhang Q., Xiang J., Lizhen Z., Zhu X., Van der Werf W. and Duan L. - Optimizing soaking and germination conditions to improve gamma-aminobutyric acid content in japonica and indica germinated brown rice, Journal of Functional Foods, 10 (2014) 283-291.




How to Cite

Lai, D. Q., Ngo, A. T., Le, P. T. K., Nguyen, D. H., & Nguyen, L. Q. (2019). TECHNICAL ASSESSMENT OF GAMMA-AMINO BUTYRIC ACID (GABA) PRODUCTION FROM RICE BRAN. Vietnam Journal of Science and Technology, 57(3B), 137.



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