OPTIMIZATION OF CONDITIONS FOR CAROTENOIDS EXTRACTION FROM SHRIMP WASTE USING ORGANIC SOLVENT
Keywords:Alcalse, Carotenoid, Penaeus vannamei, shrimp waste, extraction.
AbstractIn this study, factors affecting the extraction yield of carotenoids from dry shrimp waste by organic solvents such as ratio of hexane / acetone, ratio of solvent / shrimp waste, extraction temperature, extraction time, extraction method such as dynamic or static have been studied. The results showed that the solvent ratio hexane: acetone = 3: 1 gave the highest carotenoid yield. In this ratio of solvent’s mixture, the yield reached highest at temperature 60 °C for 2 hours, which was 44,64 µg / g raw shrimp waste (d.b.) (ratio of solvent to raw material 3/1). Ultrasound or vortexing gave higher extraction yield than in static conditions, which was 1.5- to 1.8- fold increase, respectively. At the ratio of solvent: dried shrimp = 4: 1, the amount of carotenoid recovered at 60°C for 2 hours reached 57.4 µg / g. However, if the shrimp waste was hydrolyzed with Alcalase at 50°C for 4 hours before extraction by solvent, the amount of carotenoid recovered achieved 149 µg / g of raw material
N.M. Sachindra, N. Bhaskar, and N.S. Mahendrakar. Recovery of carotenoids from shrimp waste in organic solvents. Waste Management. 26 (2006) 1092-1098.
N.M. Sachindra and N.S. Mahendrakar. Process optimization for extraction of carotenoids from shrimp waste with vegetable oils. Bioresource Technology. 96 (2005) 1195-1200.
A.P. Sánchez-Camargo, H.A. Martinez-Correa, L.C. Paviani, and F.A. Cabral. Supercritical CO2 extraction of lipids and astaxanthin from Brazilian redspotted shrimp waste (Farfantepenaeus paulensis). The Journal of Supercritical Fluids. 56 (2011) 164-173.
N. Mezzomo, J. Martínez, M. Maraschin, and S.R.S. Ferreira. Pink shrimp (P. brasiliensis and P. paulensis) residue: Supercritical fluid extraction of carotenoid fraction. The Journal of Supercritical Fluids. 74 (2013) 22-33.
C.M. Babu, R. Chakrabarti, and K.R. Surya Sambasivarao. Enzymatic isolation of carotenoid-protein complex from shrimp head waste and its use as a source of carotenoids. LWT - Food Science and Technology. 41 (2008) 227-235.
S. Lee, S. Roh, K. Park, and K.-R. Yoon. Effective extraction of astaxanthin pigment from shrimp using proteolytic enzymes. Biotechnology and Bioprocess Engineering. 4 (1999) 199-204.
S.T. Trang, L.Q. Vũ, and N.D.B. Huỳnh. Ảnh hưởng của thời gian và nhiệt độ bảo quản đến hàm lượng ẩm, protein và sataxanthin trong đầu tôm. Tạp chí Khoa học - Công nghệ Thủy sản 4 (2015) 78-84.
N. Mezzomo, B. Maestri, R.L. dos Santos, M. Maraschin, and S.R.S. Ferreira. Pink shrimp (P. brasiliensis and P. paulensis) residue: Influence of extraction method on carotenoid concentration. Talanta. 85 (2011) 1383-1391.
U.N. Ushakumari and R. Ramanujan. Astaxanthin from shrimp shell waste International Journal of Pharmaceutical chemistry research 1 (2012) 1-6.
G.T. Kjartansson, S. Zivanovic, K. Kristbergsson, and J. Weiss. Sonication-Assisted Extraction of Chitin from Shells of Fresh Water Prawns (Macrobrachium rosenbergii). Journal of Agricultural and Food Chemistry. 54 (2006) 3317-3323.
R. Chakrabarti. CAROTENOPROTEIN FROM TROPICAL BROWN SHRIMP SHELL WASTE BY ENZYMATIC PROCESS. Food Biotechnology. 16 (2002) 81-90.
Authors who publish with Vietnam Journal of Science and Technology agree with the following terms:
- The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
- The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).