RESEARCH ON PHYTOCHEMICAL AND ALFA-GLUCOSIDASE INHIBITORY ACTIVITY OF ETHYL ACETATE FRACTION OF RUELLIA TUBEROSA
Keywords:Rullia tuberosa L., flavonoid, anthraquinone glycoside, α-glucosidase inhibitory activity
Rullia tuberosa L. is a folk remedy in the treatment of diabetes mellitus, anti inflammation and diuretic agent. However, its hypoglycemic activity has not been investigated so far. In the present study, the antidiabetic activity of the n-hexane, ethyl acetate and methanol fraction of crude ethanolic extract of this plant were investigated. Our preliminary results showed that the crude ethanol extract as well as ethyl acetate and methanol fractions of Ruellia tuberosa showed α-glucosidase inhibitory activity with IC50 of 15.84, 4.73 and 8.27 µg/ml, respectively. From the ethyl acetate extract of whole plant of Rullia tuberosa, four compounds, including rutin (1), syringaresinol (2), catechin (3) and pulmatin (4) were isolated and identified by column chromatography. Their structures were elucidated by spectroscopic method including MS, 1D and 2D- NMR and comparison with the literature values.
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