EFFECT OF RICE BRAN OIL ON MYCELIAL BIOMASS PRODUCTION, BIOSYNTHESIS AND BIOACTIVITIES OF POLYSACCHARIDES BY OPHIOCORDYCEPS SINENSIS FUNGUS
Ophiocordyceps sinensis (syn. Cordyceps sinensis), which is an entomopathogenic fungus, is widely known as “Dong Chong Xia Cao” in Traditional Chinese Medicine. It continues to attract scientists’ attention due to the diversity of bioactive compounds such as polysaccharides, cordycepin, adenosine, amino acids, peptides, and ergosterol. Among them, exopolysaccharides (EPS) from the culture broth has possessed many precious bioactivities, for example, immunomodulatory, antitumor, antioxidant activity, and etc. Interestingly, previous studies have demonstrated that plant oils have positive affects to the growth and biosynthesis of secondary metabolites of medicinal fungi. Therefore, the aim of this study is to enhance the biomass growth and EPS biosynthesis of O. sinensis fungus by implementing rice bran oil (RBO) at various concentrations from 1% to 8% (v/v) into the culture medium. The results illustrated that, in the RBO-added media, the biomass of mycelial and EPS production increased significantly compared to non-RBO medium. In particular, biomass yield was 26.6 g/L in medium within 6% RBO, and EPS production was 4.62 g/L in medium within 5% RBO. Importantly, inhibition of xanthine oxidase activity of the IPS and EPS crudes from the (2, 8% respectively). RBO-supplemented broths rose considerably compared to the control EPS. Besides, at 5% of RBO, the anti-inflammatory activity of EPS was highest with IC50 = 824.947 (µg/ml). In addition, the minimum IC50 value of IPS was 1529.061 (μg/ml) when added to the culture medium 4% RBO. In conclusion, these data have exposed evidence that the RBO was potential to dramatically exhibit the biomass production and EPS biosynthesis of O. sinensis fungus and improve their bioactivities.
Authors who publish with Vietnam Journal of Science and Technology agree with the following terms:
- The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
- The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).