EXTRACTION OF POLYSACCHARIDES FROM LINGZHI BY ULTRASONIC-ASSISTED ENZYMATIC METHOD
AbstractThe medicinal values of polysaccharides (PS) in Lingzhi have been shown to lie in many anti-cancer effects and good benefits for human health. Lingzhi, which is rich in healthy PS, has been used more and more commonly in Vietnam in recent years. In the present work, ultrasonic-assisted enzymatic extraction (UAEE) was used for extraction of PS from Lingzhi. The experiments were conducted according to a Box-Behnken design (BBD), with four independent variables: solvent temperature, ultrasonic power, pH, and ultrasonic irradiation time. The results showed that the best adequate extraction conditions were extraction time of 144 min, extraction temperature of 55°C, ultrasonic power of 240W, pH 7.9, and ultrasonic irradiation time of 144 min. Under these conditions, the predicted optimal yield was 3.716%. Whereas by following the optimized conditions, the experimental yield of PS was 3.65% ± 0.14, which was in good agreement with that of the prediction. Compared to the hot water extraction (HWE) method, ultrasonic-assisted extraction (UAE) method and enzyme-assisted extraction (EAE) method, the yield of PS obtained by UAEE was favorable. The PS yield obtained by HWE, and EAE were 1.96% and 3.10%, respectively. These results demonstrated that UAEE was an appropriate and effective extraction of polysaccharides from Lingzhi.
Authors who publish with Vietnam Journal of Science and Technology agree with the following terms:
- The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
- The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).