FATTY ACID PROFILE AND NUTRITION VALUES OF MICROALGA (Thalassiosira pseudonana) COMMONLY USED IN WHITE SHRIMP (Litopenaeus vannamei) CULTURING
AbstractMicroalga Thalassiosira pseudonana is an important feed item in the aquaculture industry nowadays and its biomass is essential for white leg shrimp seeding production. Microalga T. pseudonana were grown under optimal conditions (salinity of 30 - 31 ‰; pH 7.5 - 8.5; temperature 25 - 27 oC; light intensity 5.5 klux with light: dark period 12:12 h; alkalinity 150 - 180 ppm; media of AGP 20 %; initial cell density 0.2 x 106 cells/ml; 24 hour aeration mode) and harvested during log phase after 6 days of cultivation. The obtained results showed that microalga T. pseudonana possess both EPA (eicosapentaenoic acid, C20:5 n-3) and DHA (docosahexaenoic acid, C22:6 n-3) fatty acids. Particularly, its fatty acid composition is mainly C16:0 (38.060 % compared to total fatty acid - TFA), C16:2n - 4 (12.850 % TFA), 16:3n - 3 (8.873 % TFA), C20:5n - 3 (16.423 % TFA) and 22: 6 (n - 3) (1.650 % TFA). Also, the C16:1n - 7 (6.147 %), C16:1n - 9 (4.737 % TFA), C18:1n - 7 (1.370 % TFA), C18:1n - 9 (2.063 % TFA) and C20:1n - 7 (0.677 % TFA) which were obtained those play a crucial role in aquaculture activities. Additionally, the total content of SFAs, MUFFAs and PUFFAs were 42.539, 14.994 and 42.443 % TFA, respectively. The protein, carbohydrate, chlorophyll a, carotenoids, lipid contents was reach 13.20 ± 0.01 %, 9.96 ± 0.12 %, 1.01 ± 0.13 %, 0.15 ± 0.01 %, 20.79 ± 0.24 % of dry cell weight, respectively. Moreover, T. pseudonana biomass contains a large variety of minerals and micronutrients such as Na (27.80 %/kg), K (0.93 %/kg), I (55.10 mg/100g), Fe (222.74 mg/kg), Cu (1.96 mg/kg), Mn (3.11 mg/kg), Mg (2.52 %/kg), Ca (0.97 %/kg), Co (< 0.10 mg/kg) and undetectable B. Importantly, the heavy metals contents such as Cd, Pb, As and Hg was met to the Vietnam standard for aquaculture feed.
Derner R. B., Ohse S., Villela M., Carvalho S. M., de Fett R. - Microalgas produtos e aplicacões. Ciência Rural, 36 (6) (2006) 1959-1967.
Brown M. R. - Nutritional value and use of microalgae in aquaculture. In: Cruz - Suárez, L.E., Ricque-Marie, D., Tapia - Salazar, M., Gaxiola - Cortes, M. G., Simoes, N. (Eds). Advances en Nutrición VI. Memorias del VI Simposium internacional de Nutrición Acúicola. 3 al 6 de Septiembre del 2002. Cancún, Quintana Roo, Mexico (2002).
Tonon T., Harvey D., Larson T. R. and Graham I. A. - Long chain polyunsaturated fatty acid production and partitioning to triacylglycerols in four microalgae. Phytochemistry 61 (2002) 15–24. doi:10.1016/S0031-9422(02)00201-7. PMID:12165297.
Mansour M. P., Frampton D. M. F., Nichols P. D., Volkman J. K., Blackburn S. I. - Lipid and fatty acids yield of nine stationary-phase microalgae: Applications and unusual C-24-C-28 polyunsaturated fatty acids. J Appl Phycol 14 (2005) 255-265.
Milledge J. J. - Commercial application of microalgae other than as biofuels: A brief review. Rev Environ Sci Bio-Technol 10 (2011) 31-41.
Bligh E. G. and Dyer W. J. - A rapid method of total lipid extraction and purification. Can. J. Biochem. Physio., 37 (8) (1959) 911-917.
Dang Diem Hong, Hoang Minh Hien, Nguyen Dinh Hung, Hoang Sy Nam, Hoang Lan Anh, Ngo Hoai Thu, Dinh Khanh Chi - Study on DHA biosynthesis from Labyrinthula, Schizochytrium – a new heterotrophic marine algae and their utilizations. J Sci Technol 45 (1B) (2007) 144-154.
Lichtenthale H. K., - Chlorophylls and carotenoids pigments of photosynthetic. Methods Enzymol., 148 (1994) 350-382.
Wellburn A. R., - The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of diffirent resolution. J. Plant Physiol., 144 (1994) 307-313.
Hong, D. D. & Hien, H. M. - Nutritional analysis of Vietnamese seaweeds for food and medicine. BioFactors 22 (2004) 323–5.
Horwitz, W. - Official Method of Analysis of AOAC International. Published by AOAC International, Gaithersburg, Maryland, (2000) 2200 pp.
Brown M. R., Jeffrey S. W., Wolkman J. K., Dunstan G. A. - Nutritional properties of microalgae for mariculture. Aquaculture 151 (1997) 315-331.
Mata T. M., Martins A. A., Cetano N. S. - Microalgae for biodiesel production and other applications: a review. Renewable and Sustainable Energy Reviews 14 (2010) 217-232.
Ohse S., Derner B. R., Ozório Á. R., Corrêa G. R., Furlong B. E., Cunha R. C. P. - Lipid content and fatty acid profiles in ten species of microalgae. IDESIA (Chile) Volumen 33 Nº 1 (2015) 93-101.
Miao X., Wu Q. - High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides. Journal of Biotechnology 110 (2004) 85-93.
Langdon C. J., Waldock M. J. - The effect of algal and artificial diets on the growth and fatty acid composition of Crassostreagigas spat. Journal of the Marine Biological Association, United Kingdom 61 (1981) 431-448.
Sargent J. R., McEvoy L. A., Bell J. G. - Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds. Aquaculture 155 (1997) 117-127.
Pratoomyot J., Srivilas P. and Noiraksar T. - Fatty acids composition of 10 microalgal species. Songklanakarin J. Sci. Technol., 27 (6) (2005) 1179-1187.
Widianingsih, Hartati R., Endrawati H. and Mamuaja J. - Fatty acid composition of marine microalgae in Indonesia. Journal of tropical biology and conservation 10 (2013) 75-82.
Lang I., Hodac L., Friedl T., Feussner I. - Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biol 11 (2011)124.
Anaman V., Unprasert N., Thongrod S. and Khachantongsuwan S. - Effect of levels and sources of dietary lipid on growth and fatty acids compositions in the liver of Notopterus chitala (Hamilton). J. Fisheries., 53 (2) (2000) 121-131.
Tamtin T., Thongrod S., Chayarat Ch. and Boonyaratpalin M. - Optimum dietary n-3 to n-6 fatty acids ratio for abalone growth (Haliotis asinina, LINNE). Technical paper no. 50/2547. Coastal Aquatic Feed Research Institute. Sri Racha. Chonburi (2004).
Richmond A. - Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Cornwall: Blackwell Publishing (2004) 566 p.
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