THE PROTECTIVE EFFECT OF SOME FRACTIONS AND ISOLATED COMPOUNDS FROM EUPHORBIA HIRTA L. ON PANCREATIC β-CELLS MIN6

Authors

  • Bach Thi Le Department of Chemistry, College of Natural Sciences, Can Tho University.
  • Dung Tien Le Institute of Applied Materials Science, Vietnam Academy of Science and Technology.
  • Tuan Trong Nguyen Department of Chemistry, College of Natural Sciences, Can Tho University.
  • Thanh Quoc Chau Nguyen Department of Chemistry, College of Natural Sciences, Can Tho University.
  • Leclercq Joëlle Quetin Laboratoire de Pharmacognosie, LDRI, Université catholique de Louvain.
  • Buu Hue Thi Bui Department of Chemistry, College of Natural Sciences, Can Tho University.

DOI:

https://doi.org/10.15625/2525-2518/56/4A/12753

Keywords:

Euphorbia hirta L., ER Stress, MIN6.

Abstract

Euphorbia hirta L., a kind of herbs belonging to family Euphorbiaceae, has been found a lot in the Mekong Delta. From the ethyl acetat and methanol fractions of this plant, four pure compounds were isolated and identified their chemical structure by spectroscopic techniques including quercitrin (1), luteolin (2), quercetin (3) and caffeic acid (4). The effect of crude ethanolic extract and some fractioned extracts as well as four isolated compounds from E  hirta L., collected in the Mekong Delta, on endoplasmic reticulum stress-induced cell death in mouse β-cell lines was investigated. The results showed that methanol fraction and a isolated compound, quercitrin gave strong cell-protective effect with the cell viability of 69% and 78% at the dose of 100 µg/mL and 10 µg/mL, respectively. This proves that E hirta L. might have anti-diabetic effects and worth conducting further pharmacological investigations related to type 2 diabetes mellitus.

 

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References

Umut O¨ zcan, Erkan Yilmaz, Lale O¨zcan, Masato Furuhashi, Eric Vaillancourt, Ross O. Smith, Cem Z. Go¨rgu¨ n, Go¨khan S. Hotamisligil - Chemical chaperones reduce er stress and restore glucose homeostasis in a mouse model of type 2 diabetes, Science, 313 (2006) 1137–1140.

De´cio L. Eizirik, Alessandra K. Cardozo, and Miriam Cnop - The role for endoplasmic reticulum stress in diabetes mellitus, Endocrine reviews, 29 (1) (2008) 42–61.

Amedeo Vetere1, Amit Choudhary, Sean M. Burns and Bridget K. Wagner - Targeting the pancreatic beta-cell to treat diabetes, Nature Reviews-Drug Discovery, 13 (2014) 278–289.

Do Tat Loi, These medicinal plants and herbs in Vietnam - Publishing Medicine (in Vietnamese), (2014) 199-200.

Kumar, S., Malhotra, R, and Kumar, D.- Euphorbia hirta: its chemistry, traditional and medicinal uses, pharmacological activities, Pharmacognosy reviews, 4 (7) (2010) 58.

Hideyuki Tominaga, Munetaka Ishiyama, Fumio Ohseto, Kazumi Sasamoto, Tomoyuki Hamamoto, Keiji Suzuki and Masami Watanabe - A water-soluble tetrazolium salt useful for colorimetric cell viability assay, Analytical Communications, 36 (1999) 47–50.

O.A. Eldahshan - Isolation and structure elucidation of phenolic compounds of carob leaves grown in egypt, Current Research Journal of Biological Sciences, 3 (1) (2011) 52-55.

Mohamed Ali A. Alwahsh, Melati Khairuddean and Wong Keng Chong Chemical - Constituents and antioxidant activity of teucrium barbeyanum aschers, Records of Natural Products, 9 (1) (2015) 159-163.

V. Sai Saraswathia, D. Saravananb and K. Santhakumar - Isolation of quercetin from the methanolic extract of lagerstroemia speciosa by HPLC technique, its cytotoxicity against mcf-7 cells and photocatalytic activity, Journal of Photochemistry & Photobiology, B: Biology (2017).

Guoyu Shi, Meixia Xu, Wenjuan Duan, Lei Fang, Wei Liu, Xiao Wang and Yongqing Zhang - Chemical constituents from trichosanthis pericarpium, Asian Journal of Chemistry, 26 (15) (2014) 4626-4630.

Butler, A. E., Janson, J., Bonner-Weir, S., Ritzel, R., Rizza, R. A., and Butler, P. C. - Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes, Diabetes Metabolism Researche and Reviews, 52 (2003) 102–110.

Sunil Kumar, Rashmi and D Kumar - Evaluation of antidiabetic activity of Euphorbia hirta Linn. in streptozotocin – induced diabetic mice, Indian Journal of Natural Products and Resources, 1 (2) (2010) 200-203.

Anup Kumar Maurya, Smriti Tripathi, Zabeer Ahmed, Ram Kumar Sahu - Antidiabetic and antihyperlipidemic effect of Euphorbia hirta in streptozotocin induced diabetic rats, Der Pharmacia Lettre, 4 (2) (2012) 703-707.

Sorimuthu P. Subramanian, Subramanian Bhuvaneshwari and Gopalan S. Prasath - Antidiabetic and antioxidant potentials of Euphorbia hirta leaves extract studied in streptozotocin-induced experimental diabetes in rats, General Physiology and Biophysics, 30 (2011) 278–285.

Ranganathan Babujanarthanama , Purushothaman Kavithaa , Moses Rajasekara Pandian - Quercitrin, a bioflavonoid improves glucose homeostasis in streptozotocin-induced diabetic tissues by altering glycolytic and gluconeogenic enzymes, Fundamental & Clinical Pharmacology, 24 (2010) 357–364.

Mona F. Mahmoud, Noura A. Hassan, Hany M. El Bassossy, Ahmed Fahmy - Quercetin protects against diabetes-induced exaggerated vasoconstriction in rats: effect on low grade inflammation, PLOS ONE, 8 (5) (2013) 1-11.

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Published

2018-10-19

How to Cite

Le, B. T., Le, D. T., Nguyen, T. T., Nguyen, T. Q. C., Joëlle Quetin, L., & Bui, B. H. T. (2018). THE PROTECTIVE EFFECT OF SOME FRACTIONS AND ISOLATED COMPOUNDS FROM EUPHORBIA HIRTA L. ON PANCREATIC β-CELLS MIN6. Vietnam Journal of Science and Technology, 56(4A), 163. https://doi.org/10.15625/2525-2518/56/4A/12753

Issue

Section

The 6th National Workshop on Research and Development of Natural Products