A GREEN AND SIMPLE SYNTHESIS OF CHITOSAN/Ag NANOCOMPOSITES AND STUDY FOR THEIR ANTIBACTERIAL ACTIVITY ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI
Keywords:Chitosan/silver nanocomposites (CS/Ag NCPs), Escherichia coli (E. coli) bacteria, environmental friendly, green synthesis, Kumquat extract.
AbstractA green and simple approach has been successfully developed to synthesize chitosan/Ag nanocomposites using kumquat extract as a biological reducing agent. It indicates to be an eco-friendly and green method for the synthesis providing a cost effective and an efficient route for the chitosan/Ag nanocomposites’ synthesis. The prepared chitosan/Ag nanocomposites have been characterized by UV-vis, TEM, FTIR, and XRD. Result showed those chitosan/Ag nanocomposites have been obtained with average particle size ~15-25 nm. Moreover, the synthesized chitosan/Ag nanocomposites also showed their efficient antimicrobial activity against S. aureus and E. coli. The chitosan/Ag nanocomposite was found to have signiﬁcantly higher antimicrobial activity than its components at their respective concentrations. The presence of a small percentage (2.5 %, w/w) of metal nanoparticles in the nanocomposite was enough to signiﬁcantly enhance inactivation of S. aureus and E. coli as compared with unaltered chitosan. Thus, this eco-friendly method could be a competitive alternative to the conventional physical/chemical methods used for the synthesis of chitosan/Ag nanocomposites. Since, it has a potential to use in biomedical and cosmetic applications.
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