Rhodosporidium sp. GROWTH IN MOLASSES MEDIUM AND EXTRACTION OF ITS ASTAXANTHIN BY USING HCl

Authors

  • Tran Quang Vinh

DOI:

https://doi.org/10.15625/2525-2518/55/1A/12377

Keywords:

astaxanthin, Rhodosporidium sp., pilot, molasses medium, HCl extract.

Abstract

Astaxanthin is classified as a xanthophyll-carotenoid, which is red-orange colour and powerful antioxidant activity. In this study, astaxanthin was collected from Rhodosporidum sp. by pilot culture (10 liters). Molasses medium was investigated with urea ((NH4)2CO), magnesium sulfate heptahydrate (MgSO4.7H2O) and potassium dihydrogen phosphate (KH2PO4) at different concentrations. Astaxanthin was extracted by using chlorhydric acid (HCl) method.

The highest dried yeast biomass was 8.3682 g/l culture supernatant and astaxathin was 1.932 g/l culture supernatant by molasses medium containing 20 g/l sugar, 0.5 g/l ((NH4)2CO, 3 g/l MgSO4.7H2O and 10 % (v/v) inoculum. HCl extraction method was mixed 10 mg biomass: 1 ml HCl 0.6 N and incubated at 70 oC, 150 minutes.

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Published

2018-04-24