IN VITRO ASSESSMENT OF POTENTIAL PROBIOTIC MICROORGANISMS FOR APPLICATION IN ANIMAL FEEDING
Keywords:animal feed, digestive enzyme, antimicrobial activity, simulated gastrointestinal conditions.
This study aimed to evaluate and to select potential probiotic microorganisms. The obtained results would be further studied for application in production of animal feed. A total of 16 strains of microorganisms including 11 strains of Lactobacillus, four strains of Bacillus and a yeast strain Saccharomyces boulardii PLCP were investigated for acid production, digestive enzyme production and antimicrobial activity as well as their survival when exposed to simulated gastrointestinal conditions. The results showed that all 11 strains of Lactobacillus bacteria were capable of acid generation (in the range from 18.05 – 19.04 g/l). All four strains of Bacillus bacteria were capable of producing protease. Only Bacillus sp D7 strain was capable of producing three digestive enzymes (protease, amylase, cellulase) with hydrolyzed hollows ranged from 15.5–18.5 mm. The antibacterial activity of 9/16 test strains was positive against Salmonella Typhimurium, Staphylococcus aureus and Escherichia coli. Survivability of 15 test microorganisms in simulated gastrointestinal conditions was relatively high (c.a. 80 %). Three strains of L. acidophilus VAST, S. boulardii PLCP and Bacillus sp D7 when in mixture demonstrated a great potential as probiotics for animal.
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