CHARACTERIZATION OF CELLULASE PREPARATION OF BACILLUS SP.G4 ISOLATED FROM THE TERMITES GUT

Authors

  • Đào Thị Thanh Xuân Vinh University, 182 Le Duan, Vinh city, Nghe An

DOI:

https://doi.org/10.15625/2525-2518/54/4A/11985

Keywords:

Bacillus, Cellulase, CMCase, isolation, EDTA, SDS, Tween 80, termite gut.

Abstract

The Bacillus sp. strain G4 isolated from termites gut can produce cellulase with high activity in rice bran medium after 72 h of incubation at 37 °C. The crude enzymewas collected by centrifugation at 6,000 rpmfor 15 minutes.  The precipitation of cellulasewith ammonium sulfate 60 – 90 % saturation and acetone at the concentration 60 % – 90 % was studied. Results showed that ammonium sulfate 90 % saturation gave cellulase with highest purification factor 8.87 but the yield was only 59.9 %, whereas acetone at 90 % concentration gave highest yield 83.57 % with purification factor 4.38. CMCase activity of cellulasepreparation obtained by acetone precipitation at 90 % was optimum at pH 7 and 60 °C. Furthermore, CMCase was stable at pH 6.0 - 7.0 and at temperature lower than 50 °C. The CMCase was activated by ion Ca2+ but inhibited by Co2+, Zn2+, Cu2+, Fe2+, and was not affected by Mg2+ and Ba2+. The CMCase was inhibited by high concentration of SDS while EDTA and Tween 80 played activated role.

Downloads

Download data is not yet available.

Downloads

Published

2018-03-21

Issue

Section

Articles