OPTIMAL CONDITIONS FOR EXOPOLYSACCHARIDE PRODUCTION BY Lactobacillus plantarum T10

Authors

  • Tran Bao Khanh Faculty of Chemistry, Hue University of Science, 77 Nguyen Hue street, Hue city

DOI:

https://doi.org/10.15625/2525-2518/54/4A/11976

Keywords:

exopolysaccharide, cultivation condition, Lactobacillus plantarum, sugar.

Abstract

Exopolysaccharide (EPS) production ability of Lactobacillus plantarumT10 was studied. The supplement of some sugars (lactose, saccharose, and glucose) gave the positive effects on EPS production of L. plantarum T10, in which the addition of lactose 4 % resulted in the most efficiency for EPS yield (274.83 μg/mL). The addition of 0.4 % of yeast extract into culture medium with 4 % lactose provided the highest EPS yields compared to other nitrogen sources (peptone, beef extract), which were 378.32 mg/mL. The optimal conditions for EPS production of L. plantarum T10 in MRS broth with 4 % of lactose and 0.4 % yeast extract supplement were also studied. The results indicated that the highest EPS yield (417.11 mg/L) was obtained in the conditions of 106 CFU/ml initial cell density, temperature of 35 oC, pH 5.5 and 48 h incubation.

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Published

2018-03-21

Issue

Section

Articles