SCREENING OF PEPTIDE RECEPTOR SEQUENCES FOR AMITROL DETECTION USING CHROMATOGRAPHIC BIOPANNING
Keywords:Amitrol, phage display, chromatographic biopanning
Endocrine disrupting chemicals (EDCs) have been known as health threatening compounds and much attention has been paid in a research related with detection and/or removal from food sources and living environment. Amitrol, classified as one of EDCs, is hard to be detected and removed due to its chemical characteristics such as small molecular weight, low reactivity and high solubility. Some of organic compounds were investigated to find the corresponding binding peptides to those by biopanning protocol. However, peptides with affinity to amitrol remained to be searched for because amitrol is hard to be used as a target of free form or immobilized form in common biopanning protocol. In our experiment, amitrol was successfully immobilized on CDI monolithic column with the presence of catalyst and hence was able to be applied as a target component in biopanning. Chromatic biopanning was carried out as a fast and convenient method for the selection of peptides with high affinity to amitrol. Chemical and physical methods were combined in elution step to improve the selection efficiency for strong binders. After multiple rounds of negative screening and positive screening, high affinity peptide sequences were isolated from initial peptide libraries. Applications of these peptide sequences can be widened from bio adsorption to biosensor for environment pollution control.