INVESTIGATION OF INENZYME TREATMENTS TO ASSIST EXTRACTION OF ESSENTIAL OIL FROM THE LEAVES AND BRANCHES OF CINNAMOMUM CASSIA COLLECTED IN YEN BAI PROVINCE
Keywords:enzyme assisted extraction, cassia oil, Cinnamomum cassia, cellulase, laccase.
In order to improve the efficiency of essential oil distillation of the leaves and branches
from the cassia plant (Cinnamomum cassia (L.) J. Presl) growing in Yen Bai province, the
effects of enzyme treatments of the plant materials before distillation were investigated using
crude laccase obtained from culture medium of the fungus Ganoderma lucidum and Cellic Htech
2 (Novozymes, Denmark), a multienzyme system consisting of cellulase and xylanase. The
results showed that enzyme treatments increased oil yield and shorten distillation time. The use
of a mixture of both enzyme systems was more effective than using them separately. Under
optimal conditions, an increase in oil yield of 41.7 % was achieved, while distillation time was
shortened from 8 to 5 hours. The enzyme treatments did not change the qualitative composition
of the essential oils. However, significant changes in the percentages of cinnamic aldehyde
(69.74 % to 85.6 %) and cinnamyl acetate (17.2 % to 1.34 %) were observed.
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