SYNTHESIS OF BIOTINYLATED FERROCENE, AND ITS APPLICATION AS A REDOX PROBE FOR ELECTROCHEMICAL IMMUNOSENSOR

Authors

  • Le Huu Quynh Anh HochiminhCity University of Natural Resources and Environment, 236B Le Van Sy St., Tan Binh District, Hochiminh City, Vietnam

DOI:

https://doi.org/10.15625/2525-2518/54/2C/11873

Keywords:

immunosensor, ferrocene, redox probe, electrochemical detection, polypyrrole.

Abstract

The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene through
covalent bonding was studied. Synthesis and structure identification of Biotinylated 1 - (- 2-
aminoethyl) ferrocene were performed by 1H-NMR, 13C-NMR and MS. The ultimate goal of this
paper is to develop derivatives of ferroceneservingas an electrochemical probe that would
enhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidin
layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The
measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry
(DPV) method and underlined a variation of redox properties upon the antigen interaction. The
introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good
sensitivity of the biosensor with a detection limit of 0.16 pg.mL-1.

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Published

2018-03-19

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Section

Articles