SYNTHESIS OF BIOTINYLATED FERROCENE, AND ITS APPLICATION AS A REDOX PROBE FOR ELECTROCHEMICAL IMMUNOSENSOR
Keywords:immunosensor, ferrocene, redox probe, electrochemical detection, polypyrrole.
The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene through
covalent bonding was studied. Synthesis and structure identification of Biotinylated 1 - (- 2-
aminoethyl) ferrocene were performed by 1H-NMR, 13C-NMR and MS. The ultimate goal of this
paper is to develop derivatives of ferroceneservingas an electrochemical probe that would
enhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidin
layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The
measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry
(DPV) method and underlined a variation of redox properties upon the antigen interaction. The
introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good
sensitivity of the biosensor with a detection limit of 0.16 pg.mL-1.
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