STUDY ON PATHOGEN OF LUMINOUS DISEASE VIBRIO HARVEYI ON LARVAE AND JUVENILE OF MUD CRAB (SCYLLA SERRATA) IN HATCHERY
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DOI:
https://doi.org/10.15625/1859-3097/16/2/8456Keywords:
Larvae and juvenile of mud crab, Scylla serrata, luminous disease, bacteria.Abstract
Luminous disease is known as the most popularly dangerous disease in the hatcheries of mud crab (Scylla serrata) in northern provinces. Without any treatment, the mortality caused by this disease may reach 50 - 100% after 2 - 3 days infected. In this study, Vibrio harveyi has been identified as the main pathogen which causes the disease in larvae and juvenile of mud crab by using method for bacteria identification (bio-chemical reaction and comparision of the gene 16S rDNA) as well as artificial disease infection. In addition, we have developed successful prevention and treatment for this disease as follows: potasium permanganate, chlorine, formaline used for treatment of water, crab spawners, living food (artemia), utensils and equipment, after that one of the three different kinds of antibiotics can be used: Neomycine, Erythromycine or Doxycyclin. For prevention, the dose of 2 ppm/day can be applied for 3 consecutive beginning days of each development stage (Zoea, Megalope and juvenile), and 3 ppm/day can be applied for 3 consecutivedays for disease treatment.
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Baticados, M. C. L., and Pitogo, C. L., 1990. Chlorination of seawater used for shrimp culture. The Israeli Journal of Aquaculture-Bamidgeh, 42(4): 128-130.
Baticados, C. L., 1988. Control of luminous bacterial infection in prawn hatcheries. SEAFDEC Asian Aquaculture (Philippines). No 1, 2-4.
Hoàng Đức Đạt, 2000. Kỹ thuật nuôi cua biển. Nxb. Nông nghiệp. Tp. Hồ Chí Minh.
Lavilla-Pitogo, C. R., Leobert, D., & la Peña, D., 2004. Diseases in Farmed Mud Crabs Scylla Spp: Diagnosis, Prevention, and Control. Aquaculture Department, Southeast Asian Fisheries Development Center.
Linda L. and Mccarter, 1998. Controls Opacity of Vibrio parahaemolyticus. http://linda-mccarter@uiowa.edu.
Nguyễn Cơ Thạch, 2000. Nghiên cứu sinh sản nhân tạo cua biển loài cua Scylla serrata (Forskal, 1775). Báo cáo tổng kết đề tài cấp Bộ. Bộ Nông nghiệp và Phát triển Nông thôn. 120 tr.
Pitogo, C. L., 1988. Isolation and identification of luminous bacteria causing mortalities in Penaeus monodon hatcheries in Panay [Philippines]. SEAFDEC Asian Aquaculture (Philippines). No 1, 11 -13.
http:// webmaster@watson.wustl.edu.
West P.A., 1996. Numerical taxonomi of Vibriosis isolated from aquatic environment. Int.J syst. Bacteriol.36: 531 - 543. DOI: https://doi.org/10.1099/00207713-36-4-531