A study on bacteria associated with three hard coral species from Ninh Thuan waters by epifluorescence and most diluted culture method

Pham Thi Mien; Nguyen Kim Hanh; Nguyen Minh Hieu; Phan Minh Thu; Hoang Trung Du; Vo Hai Thi; Nguyen Trinh Duc Hieu; Le Tran Dung; Nguyen Huu Huan

doi:10.15625/1859-3097/19/2/10814

Author affiliations

Authors

Pham Thi Mien Institute of Oceanography, VAST, Vietnam
Nguyen Kim Hanh Institute of Oceanography, VAST, Vietnam
Nguyen Minh Hieu Institute of Oceanography, VAST, Vietnam
Phan Minh Thu Institute of Oceanography, VAST, Vietnam
Hoang Trung Du Institute of Oceanography, VAST, Vietnam
Vo Hai Thi Institute of Oceanography, VAST, Vietnam
Nguyen Trinh Duc Hieu Institute of Oceanography, VAST, Vietnam
Le Tran Dung Institute of Oceanography, VAST, Vietnam
Nguyen Huu Huan Institute of Oceanography, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/19/2/10814

Keywords:

Symbiotic microbes, bacteria, Acropora hyacinthus, Acropora muricata, Acropora robusta, environmental parameters, Ninh Thuan.

Abstract

Coral associated bacteria and their host are currently one of the interested issues for research and scientists worldwide. The densities of zooxanthellae and bacteria associated with three most prevalent species Acropora hyacinthus, Acropora muricata and Acropora robusta in Hang Rai, Ninh Thuan was evaluated over time by staining with SYBR Gold and direct counting with epifluorescence method. The most dominant bacteria were isolated by culture dependent method. The densities of zooxanthellae and bacteria ranged from 0.39–1.83×10⁷ cell/g, and 0.83–2.52×10⁸ cell/g, respectively. Bacterial density in the 3 months was significantly different compared to the density of the bacteria in ambient water. Total heterotrophic bacteria, comma shaped bacteria and bacillus form showed negatively correlated with pH, PO₄, while zooxanthellae showed no correlation with all factors.

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