Immobilizing Alcalase® Enzyme onto Magnetic Nanoparticles

Authors

  • Cao Xuan Huu Department of Electronics & Telecommunication Engineering, Da Nang University of Technology
  • Nguyen Bao Linh
  • Nguyen Thi Hoang Yen
  • Dang Duc Long Department of Chemistry Engineering, Da Nang University of Technology

DOI:

https://doi.org/10.15625/0868-3166/24/3S1/5464

Keywords:

magnetic nanoparticles, alkaline protease, enzyme immobilization, glutaraldehyde

Abstract

In recent years, magnetic nanoparticles (MNPs) have been applied to numerous biological systems. The nanoparticles are particularly useful in separating biological molecules due to its low price, scalable ability and very little interference. Here, MNPs, which can efficiently separate biocatalysts from reaction media by external magnet, was used to immobilize an alkaline protease (Alcalase®). Covalent attachment of the enzyme to MNPs began with the functionalization of the MNPs' surface with amines (APTES). Then, glutaraldehyde was introduced to link the MNP surface amines with enzyme surface amine residues, typically lysine. Successful covalent bonds were checked by FT-IR. Our results showed the attached enzyme did not affect superparamagnetic property of MNPs, therefore the MNPs-attached enzyme was easily recovered after the reaction. The immobilized enzyme maintained its activities after 10 times of recycle uses.

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Published

13-11-2014

How to Cite

Huu, C. X., Linh, N. B., Yen, N. T. H., & Long, D. D. (2014). Immobilizing Alcalase® Enzyme onto Magnetic Nanoparticles. Communications in Physics, 24(3S1), 121–126. https://doi.org/10.15625/0868-3166/24/3S1/5464

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Section

Papers