Optic bionanospherical probe from Gd\(_2\)O\(_3\): Yb, Er upconverting nanosphere and mAb^CD133 antibody for precise imaging label of cancer stem cell NTERA-2

Nguyen Thanh Huong, Do Thi Thao, Nguyen Trong Nghia, Nguyen Thi Nga, Pham Thi Lien, Ha Thi Phuong, Nguyen Van Nghia, Vu Duong, Tran Thu Huong, Tran Kim Anh, Le Quoc Minh\(^{\star}\)
Author affiliations

Authors

  • Nguyen Thanh Huong \(^{1}\)Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
    https://orcid.org/0000-0002-4468-8965
  • Do Thi Thao \(^{1}\)Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Nguyen Trong Nghia \(^{1}\)Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Nguyen Thi Nga \(^{1}\)Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Pham Thi Lien \(^{1}\)Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Ha Thi Phuong Medical University, Hanoi 100000, Vietnam
  • Nguyen Van Nghia Thuyloi University, Hanoi 116705, Vietnam
  • Vu Duong \(^{1}\)Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Tran Thu Huong \(^{1}\)Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam
  • Tran Kim Anh Institute of Research and Development, 3 Quang Trung, Da Nang 55000, Vietnam
  • Le Quoc Minh\(^{\star}\) \(^{1}\)Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam;
    \(^2\)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Hanoi 100000, Vietnam

DOI:

https://doi.org/10.15625/0868-3166/18226

Keywords:

Bionanoprobe, upconversion Gd2O3:Yb, Er, label, cancer stem cells NTERA-2

Abstract

Rare earth photonic nanomaterials are increasingly prominently applied in various fields of biomedicine. Currently, there is greater focus on the investigation to control the size and shape of nanomaterials, including the nanospherical form, which allows for precise labeling by only one nanoparticle. This paper demonstrates, for the first time, the construction of a biological nanospherical probe (BNSP) Gd2O3: Yb3+, Er3+/Silica/NH/mAb^CD133 for diagnostic labeling of cancer stem cells (CSCs) NTERA-2. The BNSP was constructed using highly monodisperse spheres with around 200nm uniform size of Gd2O3: 7.6% Yb3+, 1.6% Er3+. They were functionalized by an amine group-contained shell coating and conjugated with CD133 monoclonal antibody. The functionalized nanosphere Gd2O3: Yb, Er/silica/NH2 showed strong upconversion luminescence in red color upon laser excitation in the near-infrared region at 975 nm. The Gd2O3: Yb3+, Er3+/silica/NHwas carefully implemented to conjugate mAb^CD133 via a linker, glutaraldehyde, to obtain the predictable probe Gd2O3: Yb3+, Er3+/Silica/NH/mAb^CD133. Then, this BNSP was tested in vitro for its capacity to label NTERA-2 cancer stem cells. The efficient labeling based on the fluorescent immunoassay method was detected by incorporating a nanophotometer, Field Energy Scan Electron Microscopy (FESEM), and precisely determined by fluorescent microscopy. The study shows that the BNSP is highly efficient with targeting capacity and specificity in the labeling of cancer stem cells. These advanced results open up promising avenues for the development of precise imaging diagnostics in cancer cellular biomedicine, and beyond.

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Published

09-08-2023

How to Cite

[1]
H. Nguyen, “Optic bionanospherical probe from Gd\(_2\)O\(_3\): Yb, Er upconverting nanosphere and mAb^CD133 antibody for precise imaging label of cancer stem cell NTERA-2”, Comm. Phys., vol. 33, no. 3, p. 251, Aug. 2023.

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Received 06-04-2023
Accepted 23-06-2023
Published 09-08-2023