Characterization of nitrogen-fixing and IAA-producing purple non-sulfur bacteria isolated from rice fields in Nam Dinh, Vietnam

Do Thi Lien; Cung Thi Ngoc Mai; Dinh Thi Thu Hang; Tran Thi mai; Nguyen Thi Kim Tien; Le Thi Nhi Cong; Bui Thi Kim Anh; Nghiem Ngoc Minh

doi:10.15625/2615-9023/22919

Author affiliations

Authors

Do Thi Lien Institute of Biotechnology
Cung Thi Ngoc Mai Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Dinh Thi Thu Hang Gradute University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Tran Thi Mai Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Ha Noi, Vietnam
Nguyen Thi Kim Tien Phacogen Pharmaceutical Joint Stock Company, Ha Noi, Vietnam
Le Thi Nhi Cong Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Ha Noi, Vietnam
Bui Thi Kim Anh Institute of Science and Technology for Energy and Environment, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Vietnam
Nghiem Ngoc Minh Institute of Biology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Ha Noi, Vietnam

DOI:

https://doi.org/10.15625/2615-9023/22919

Keywords:

IAA production, fixing molecular nitrogen, Rhodopseudomonas palustris, Rhodobacter capsulatus, Purple photosynthetic bacteria.

Abstract

This study aimed to select purple photosynthetic bacteria (PPB) isolated from various paddy fields, including saline fields in Giao Thuy and paddy fields in Y Yen, based on their biofertilizer properties. Among 24 PPB isolates, strains GT10 and Y11 exhibited the highest efficiency in indole-3-acetic acid (IAA) production and nitrogen fixation. GT10 colonies were circular and convex, with smooth surfaces, reddish brown, diameter: 0.9-1.2 mm. The cell suspension appeared reddish-brown. Colonies of strain Y11 were spread out, reddish brown, slimy, diameter: 1.8-2.2 mm, with a purple-colored cell suspension. GT10 cells were oval-shaped (0.9-1.25 μm long, 0.6-0.7 μm wide) and reproduced by binary fission. In contrast, Y11 cells were rod-shaped (1.4-1.58 μm long, 0.36-0.441 μm wide) and reproduced by budding. Elemental sulfur globules were not observed in either strain. Both were Gram-negative and contained bacteriochlorophyll a as their primary photosynthetic pigment. The GT10 strain was able to use carbon sources such as acetate, propionate, lactate, succinate, formate, citrate, mannitol, sorbitol, glycerol, glutamate, isopropanol, sulfide, but not tartrate, methanol, or ethanol. These characteristics are consistent with the genus Rhodobacter. Y11 strain was able to use carbon sources such as acetate, propionate, lactate, succinate, formate, citrate, mannitol, sorbitol, glycerol, glutamate, or sulfide, but did not utilize tartrate, methanol, ethanol, or isopropanol. These capacities are similar to those of the Rhodopseudomonas species. Based on 16S rDNA sequencing, strain Y11 was identified as Rhodopseudomonas palustris, while strain GT10 was Rhodobacter capsulatus. The combined application of GT10 and Y11 strains may offer a sustainable biofertilizer alternative to chemical fertilizers in rice cultivation.

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References

Ahmad F., Ahmad I., Khan M. S., 2005. Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turk J Biol, 29: 29–34.

Bui Ba Bong, 2013. “Foreword” Proceedings of the National Workshop on Solutions to Improve the Efficiency of Fertilizer Management and Use in Vietnam. Agriculture Publishing House, Ho Chi Minh City: 3–5.

Cheng Y., Brantner S., Christine A., Tsapin A., Collins M. L., 2000. Role of the H protein in assembly of the photochemical reaction center and intracytoplasmic membrane in Rhodospirillum rubrum. J. Bacteriol., 182(5): 1200−1207.

Choudhury A. T., Kennedy I. R., 2004. Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biol Fertil Soils, 39: 219–227.

Ercal N., Orhan H. G., Burn N. A., 2001. Toxic metals and oxidative stress Part I: Machanisms involved in metal induced oxidative damage. Curr. Top Med. Chem., 1: 529–539.

Girija K. R., Sasikala Ch., Ramana Ch. V., Spröer C., Takaichi S., Thiel V. & Imhoff J. F., 2010. Rhodobacter johrii sp. nov., an endosporeproducing cryptic species isolated from semi-arid tropical soils. Int J Syst Evol Microbiol, 60: 2099–2107.

Gutierrez J., Atulba S. L., Kim G., Kim P.J., 2014. Importance of rice root oxidation potential as a regulator of CH4 production under water logged conditions. Biol Fert Soils, 50(5): 861–868.

Harada N., Nishiyama M., Matsumoto S., 2001. Inhibition of methanogens increases photo- dependent nitrogenase activities in anoxic paddy soil amended with rice straw. FEMS Microbiol Ecol, 35(3): 231–238.

Harada N., Nishiyama M., Otsuka S., Matsumoto S., 2005. Effects of inoculation of phototrophic purple bacteria on grain yield of rice and nitrogenase activity of paddy soil in a pot experiment. Soil Sci Plant Nutr, 51(3): 361–367.

Hoang Kim Chi, Nguyen Đinh Tuan, Tran Ho Quang, Nguyen Thanh Lam, Le Huu Cuong, Tran Thi Hong Ha, Le Mai Huong, Tran Thi Nhu Hang, 2019. Screening and study of some strains of growth-promoting rhizosphere bacteria isolated from turmeric (Curcuma longa L) in Vietnam. Vietnam Journal of Science and Technology, 62(5): 54–59.

Imhoff J. F., Trueper H. G., 1989. Purple non-sulfur bacteria (Rhodospirillaceae Pfennig and Trueper 197, 17AL), P: 1438-1680. In: Staley JT; Bryant M P; Pfennig N, and Holt JG. (eds.). Bergey’ manual of Systematic Bacteriology. Vol. 3. Williams and Wilkins. Baltimore.

Kantachote D., Salwa T., Kamontam U., 2005. The potential use of anoxygenic photosynthetic bacteria for treating latex rubber sheet wastewater. Electron. J. Biotechnol., 8(3): 314–323.

Kantha T., Chaiyasut C., Kantachote D., Sukrong S., Muangprom A., 2010. Selection of photosynthetic bacteria producing 5- aminolevulinic acid from soil of organic saline paddy fields from the Northeast region of Thailand. Afr. J. Microbiol. Res., 4(17): 1848–1855.

Kim M. K., Choi K. M., Yin C. R., Lee K. Y., Im W. T., Lim J. H., Lee S. T., 2004. Odorous swine wastewater treatment by purple non-sulfur bacteria, Rhodopseudomonas palustris isolated from eutrophicated ponds. Biotech. Lett., 26(10): 819–822.

Koh R. H., Song H. G.,2007. Effects of application of Rhodopseudomonas sp. on seed germination and growth of tomato under axenic conditions. J. Microbiol. Biotechnol., 17: 1805–1810.

Kolev N. I., 2007. Solubility of O2, N2, H2 and CO2 in water. In: Kolev NI, editor. Multiphase flow dynamics 3: turbulence, gas absorption and release, diesel fuel properties. Heidelberg: Springer: 185–214.

Larsen M., Santner J., Oburger E., Wenzel W. W., Glud R. N., 2015. O2 dynamics in the rhizosphere of young rice plants (Oryza sativa L.) as studied by planar optodes. Plant Soil, 390(1): 279–92.

Masepohl B., Kranz R. G., 2009. Regulation of nitrogen fixation. In: Hunter CN, Daldal F, Thurnauer M. C., Beatty J. T., editors. The purple phototrophic bacteria. Dordrecht: Springer: 759–75.

Masepohl B., Kranz R.G., 2009. Regulation of nitrogen fixation. In: Hunter CN, Daldal F, Thurnauer MC, Beatty JT, editors. The purple phototrophic bacteria. Dordrecht: Springer: 759–775.

Montano G. L., Chan J. S., Jarabelo E. R, , Pastor A. B. I, Cruz TEED., 2009. Isolation and characterization of purple nonsulfurbacteria (PNSB) from a rice paddy soil in bulacan, philippines. Philippine Journal of Systematic Biology, 3: 57–67.

Nguyen Thi Thu Hang, Nguyen Thi Thuy, 2015. Selection of Azotobacter bacteria with the ability to fix nitrogen and synthesize IAA. Journal of Forestry Science and Technology, No. 4.

Nookongbut P., Kantachote D., Megharaj M., Naidu R., 2018. Reduction in arsenic toxicity and uptake in rice (Oryza sativa L.) by As-resistant purple nonsulfur bacteria. Environ Sci Pollut Res, 25: 36530–36544.

Olivares J., Bedmar E. J., Sanjuan J., 2013. Biological nitrogen fixation in the context of global change. Mol Plant Microbe Interact, 26(5): 486–494.

Panwichian S., Kantachote D., Wittayaweerasak B., Mallavarapu M., 2010a. Isolation of purple nonsulfur bacteria for the removal of heavy metals and sodium from contaminated shrimp ponds. Electron. J. Biotechnol., 13(4): 12. doi: 10.2225/vol13-issue4-fulltext-8.

Panwichian S., Kantachote D., Wittayaweerasak B., Mallavarapu M., 2010b. Factors affecting immobilization of heavy metals by purple nonsulfur bacteria isolated from contaminated shrimp ponds. World J. Microbiol. Biotechnol., 26: 2199–2210.

Pfennig N., Trueper H. G., 1992. Characterization and identification of the Anoxygenic Phototrophic Bacteria. In: The Prokaryote: a handbook of on habitats, isolation and identification of bacteria. Springer- Verlag, Berlin: 299–311.

Pham Thi Ngoc Lan, Le Thi Thanh Xuan, Ngo Thi Bao Chau, 2020. Isolation and selection of nitrogen-fixing bacteria from vegetable soil in Pleiku city, Gia Lai province, Journal of Science and Technology, University of Science, Hue University, 17(2).

Raymond J., Siefert J. L., Staples C. R., Blankenship R. E., 2004. The natural history of nitrogen fixation. Mole Biol. Evol., 21: 541–555.

Sakarika M., Spanoghe J., Sui Y.,Wambacq E., Grunert O., Haesaert G., Spiller M., Vlaeminck S.E., 2020. Purple non-sulphur bacteria and plant production: Benefits for fertilization, stress resistance and the environment. Microb. Biotechnol., 13: 1336–1365.

Sakpirom J., Kantachote D., Nunkaew T., Khan E., 2017. Characterizations of purple non-sulfur bacteria isolated from paddy fields, and identification of strains with potential for plant growth-promotion, greenhouse gas mitigation and heavy metal bioremediation. Res Microbiol, 168(3): 266–275.

Sasikala G. H., Ramana C. H. V., 1995. Biotechnological potentials of anoxygenic phototrophic bacteria. I. Production of Single Cell Protein, vitamins, ubiquinones, hormones and enzymesand use in waste treatment. Advan. Appl. Microbiol., 41: 173–226.

Spaepen S., Vanderleyden J., Remans R., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev, 31(4): 425–428.

Thai Thanh Duoc & Nguyen Huu Hiep, 2022. Isolation and identification of nitrogen-fixing bacteria from corn root zone soil in the Mekong Delta. Can Tho University Science Journal, 58(2B): 160–171.

Torres-Rubio M. G., Valencia-Plata S. A., Bernal-Casttilo J., Martinez-Nieto P., 2000. Isolation of Enterobacteria, Azotobacter sp. and Pseudomonas sp., Producers of Indole-3-Acetic Acid and Siderophores, from Colombian Rice Rhizosphere. Revista Latinoamericana de Microbiología, 42: 171–176.

Truong Hop Tac, 2009. The impact of fertilizer use on the environment. http://www.agroviet.gov.vn/Pages/news_detail.aspx/NewsId=7877& Page=4.

Vatsala T. M., Rekha R., & Srividhya R., 2011. Novel substrate (algal protein) for cultivation of Rhodospirillum rubrum. Indian Journal of Experimental Biology, 49(10): 773.

Wang M. C., Yang C. H., 2003. Type of fertilizer applied to a paddy-upland rotation affects selected soil quality attributes. Geoderma: 114.

Watanabe K., Tanaka T., Hotta Y., Kuramochi H., Takeuchi Y., 2000. Improving salt tolerance of cotton seedling with 5-aminolevulinic acid. Plant Growth Regul, 32: 99–103.