Extraction of Lycopene from Gac Fruit (Momordica cochinchinensis Spreng) and Preparation of Nanolycopene

Ho Thi Oanh, Hac Thi Nhung, Nguyen Duc Tuyen, Lai Thi Kim Van, Trinh Hien Trung, Hoang Mai Ha

Abstract


In this work, we extracted high purity lycopene from dried Gac (Momordica Cochinchinensis) aril using organic solvents. We also succeeded in the preparation of nanolycopene. The effect of temperature on the drying of Gac aril and suitable solvents on the extraction of lycopene has been investigated. The results showed that the suitable drying temperature for Gac aril was 60-70 oC. The suitable solvents for lycopene extraction were dichloromethane or chloroform. The lycopene content in dried Gac aril is about 0.28-0.46 %. Nanolycopene was prepared successfully by freeze-dried method, with relatively small particles size of 40-60 nm. Nanolycopene is relatively stable in the inert environment in the presence of antioxidants such as butylated hydroxytoluene (BHT).

Keywords. Momordica cochinchinensis Spreng, Gac Fruit, lycopene, nanolycopene, extraction.

Keywords


Momordica cochinchinensis Spreng, Gac Fruit, lycopene, nanolycopene, extraction

Full Text:

PDF

References


Vo Van Chi. Tu Dien Cay Thuoc Viet Nam [A Dictionary of Medicinal Plants of Vietnam], Nha Xuat Ban Y Hoc, Ho Chi Minh City, (1997).

Do Tat Loi. Nhung Cay Thuoc va Vi Thuoc Viet Nam [Medicinal Plants and Drugs of Vietnam], Nha Xuat Ban Khoa Hoc va Ky Thuat, Hanoi (1991).

Pham Gia Tien, F. Kayama, F. Konishi, H.Tamemoto, K. Kasono, Nguyen Thi Kim Hung, et al. Inhibition of tumour growth and angiogenesis by water extract of Gac fruit (Momordica cochinchinensis Spreng),

International Journal of Oncology, 26(4), 881-889 (2005).

Le Khac Lam Dien, Nguyen Phuoc Minh, Dong Thi Anh Dao. Investigation different pretreatment methods and ratio of carrier materials to maintain carotenoids in gac (Momordica cochinchinensis Spreng) powder in drying process, International Journal of scientific & technology research, 2(12), 360-371 (2013).

J. Kubola, N. Meeso, S. Siriamornpun. Lycopene and beta carotene concentration in aril oil of gac (Momordica cochinchinensis Spreng) as influenced by aril-drying process and solvents extraction, Food Research International, 50, 664-669 (2013).

H.C. Mai, V. Truong, F. Debaste Carotenoids purification from gac (Momordica cochinchinensis Spreng.) fruit oil, Journal of Food Engineering, 172, 2-8 (2016).

H. Aoki, Nguyen Thi Minh Kieu, N. Kuze, K. Tomisaka and Nguyen Van Chuyen, Carotenoid Pigments in GAC Fruit, Biosci. Biotechnol. Biochem., 66(11), 2479-2482 (2002).

Le Thuy Vuong, A. A. Franke, l. J. Custer, S. P. Murphy. Momordica cochinchinensis Spreng. (gac) fruit carotenoids reevaluated, Journal of Food Composition and Analysis, 19, 664–668 (2006).

B. K. Ishida, C. Turner, M. H. Chapman, T. A. Mckeon. Fatty acid and carotenoids composition of Gac (Momordica cochinchinensis Spreng) fruit, J. Agric. Food Chem., 52, 274-279 (2004).

Dang Thi Tuyet Nhung, Pham Ngoc Bung, Nguyen Thu Ha, Thai Khanh Phong. Changes in lycopene and beta carotene contents in aril and oil of gac fruit during storage, Food Chemistry, 121, 326–331 (2010).

S. O. Kim, T. V. A. Ha, Y. J. Choi, S. H. Ko. Optimization of Homogenization–Evaporation Process for Lycopene Nanoemulsion Production and Its Beverage Applications, Journal of Food Science, 79, 8 (2014).