5-fluorouracil encapsulated CS-mPEG nanogels for controlling drug release

Nguyen Dai Hai


The purpose of this study is to synthesize and characterize poly (ethylene glycol) methyl ether (mPEG) conjugated chitosan (CS), mPEG-CS, at different ratios of 5-Fluorouracil (5-FU) delivery (5-FU-loaded mPEG-CS). The chemical cross-linking of these polymers were prepared by using 4-Nitrophenyl chloroformate reagent. The obtained mPEG-CS was characterized by Fourier transform infrared (FTIR) and proton nuclear magnetic resonance (1H NMR) spectroscopy. The 5-FU-loaded mPEG-CS particles were nearly spherical in shape with a mean diameter of 61.25 nm, determined by transmission electron microscopy (TEM). In addition, the entrapment efficiency of 5-FU was approximately 10 %. Whereas the encapsulation efficiency and loading capacity were independent of different molar ratios of mPEG, there was one factor that particularly stands out, which is 5-FU release behavior. These results indicated that mPEG-CS nanogels present the potential for controlled release of 5-FU working as a delivery system in cancer therapy.

Keywords. Poly(ethylene glycol) methyl ether, chitosan, 5-fluorouracil, nanogels, drug delivery system.


Poly(ethylene glycol) methyl ether, chitosan, 5-fluorouracil, nanogels, drug delivery system

Full Text:



M. Goldberg, R. Langer, X. Jia. Nanostructured materials for applications in drug delivery and tissue engineering, J. Biomater. Sci. Polymer. Ed., 18(3), 241-268 (2007).

O. C. Farokhzad, R. Langer. Impact of nanotech-nology on drug delivery, ACS. Nano., 3, 16-20 (2009).

Elena V. Batrakova, Alexander V. Kabanov. Pluronic block copolymers: evaluation of drug delivery concept from inert nanocarriers to biological response modifier, J. Control Release, 130, 98-106 (2008).

A. A. Sunil, N. M. Nadagouda, M. A. Tejraj. Recent advances on

chitosan-based micro- and nanoparticles in drug delivery, J. Control. Release, 100, 5-28 (2004).

Hoang D. Q., Tran T. V., Tran N. Q., Nguyen C. K., Nguyen T. H., Truong M. D., Tran D. L., Thu L. V. and Nguyen D. H. Functionalization of Fe3O4 nanoparticles with biodegradable chitosan-grafted-mPEG for paclitaxel delivery, Green Processing and Synthesis, Green Processing and Synthesis, 5, 459-466 (2016).

Hoang Phuc D., Thi Hiep N., Ngoc Phuc Chau D., Thi Thu Hoai N., Chan Khon H., Van Toi V., Dai Hai N. and Chi Bao B. Fabrication of Hyaluronan-Poly (vinylphosphonic acid)-Chitosan Hydrogel for Wound Healing Application, International Journal of Polymer Science, 2016 (2016).

P. Chan, M. Kurisawa, J. E. Chung et al. Synthesis and characterization of chitosan-339

g-poly (ethylene glycol)-folate as a non-viral carrier for tumor-targeted gene delivery, Biomaterials., 28, 540-549 (2007).

S. Y. Zhu, F. Qian, Y. Zhang et al. Synthesis and characterization of PEG modified 345

N-trimethylaminoethylmethacrylate CS nanoparticle, Eur. Polym. J., 43, 2244-2253 (2007).

A. R. Kulkarni, Y. H. Lin, H. F. Liang et al. A novel method for the preparation of

nanoaggregates of methoxy polyethyleneglycol linked chitosan, J. Nanosci. Nanotechnol., 6, 2867-2873 (2006).

Nguyen D. H., Lee J. S., Bae J. W., Choi J. H., Lee Y., Son J. Y. and Park K. D. Targeted doxorubicin nanotherapy strongly suppressing growth of multidrug resistant tumor in mice, International journal of pharmaceutics, 495, 329-335 (2015).

Nguyen D. H., Lee J. S., Choi J. H., Lee Y., Son J. Y., Bae J. W.,Lee K. and Park K. D. Heparin nanogel-containing liposomes for intracellular RNase delivery, Macromolecular Research, 23, 765-769 (2015).

Nguyen D. H., Tran N. Q. and Nguyen C. K. Tetronic-grafted chitosan hydrogel as an injectable and biocompatible scaffold for biomedical applications, Journal of Biomaterials Science, Polymer Edition, 24, 1636-1648 (2013).