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Study on conformational structure of tamarind seed polysaccharide and its sulfated derivative by light scattering method

Bui Ngo Tan, Quach Thi Minh Thu, Thanh Thi Thu Thuy


The aim of this study was to study the conformational changing when sulfated groups were introduced to the molecular chain of native tamarind seed polysaccharide (TSP). Light Scattering (LS) method was used to determine conformation of TSP and its sulfated derivative TSPS. The results indicated that both TSP and TSPS have a highly branched and more sphere-like molecule; however, after sulfation, the TSPS became more branched structure than native polysaccharide.

Keywords. Tamarind seed polysaccharide, conformation, light scattering.


Tamarind seed polysaccharide, conformation, light scattering


Lang P., Masci G., Dentini M., Crescenzi V., Cooke.

D., Gidley M. J., Fanutti C. & Reid J. S. G. Tamarind seed polysaccharide: preparation, characterisation and solution properties of carboxylated, sulphated and alkylaminated derivatives, Carbohydrate Polymers, 17, 185-198 (1992).

Shailaja T., Latha K.,Sasibhushan P., Alkabab A. M., Uhumwangho M. U. A novel bioadhesive polymer: grafting of tamarind seed polysaccharide and evaluation of its use in buccal delivery of metoprolol succinate, Der Pharmacia Lettre, 4(2), 487-508 (2012).

Gao Y., Fukuda A., Katsuraya K., Kaneko Y., Mimura T., Nakashima H. and Uryu T. Synthesis of regioselective substituted curdlan sulfates with medium molecular weights and their specific anti-HIV-1 activities, Macromolecules, 30, 3224-3228 (1997)

Burchard W. Physical Techniques for the Study of Food Biopolymers, Blackie Academic and Professional, Glasgow 151-214 (1994).

Bui Ngoc Tan, Thanh Thi Thu Thuy, Nguyen Thi Nu, Dang Vu Luong, Nguyen Tuan Anh. Structural Determination of Tamarind Seed Polysaccharide (TSP) Extracted from Tamarind Seed, Journal of Chemistry, 53(6e3), 62-64 (2015).

Bui Ngoc Tan, Quach Thi Minh Thu, Dang Vu Luong, Nguyen Tuan Anh and Thanh Thi Thu Thuy. Structure and antitumor activity of tamarind seed polysaccharide and its sulfated derivative, Journal of Science and Technology, 54(2B), 170-176 (2016).

Deveswaran R., Bharath S., Sharon Furtado, Sindhu Abraham, Basavaraj B. V., Madhavan V. Isolation and Evaluation of Tamarind Seed Polysaccharide as a Natural Suspending Agent, International Journal of Pharmaceutical & Biological Archives, 1(4), 360-363 (2010).

Lihong Fan, Lan Jiang, Yongmei Xu, Yue Zhou, Yuan Shen, Weiguo Xie, Zhongheng Long, Jinping Zhou. Synthesis and anticoagulant activity of sodium alginate sulfates, Carbohydrate Polymers, 83(4), 1797-1803 (2011).

Camila F. Becker, Jorge A. Guimarães, Paulo A.S. Mourão, Hugo Verli. Conformation of sulfated galactan and sulfated fucan in aqueous solutions Implications to their anticoagulant activities, Journal of Molecular Graphics and Modelling, 6, 391-399 (2007).

Trushar R. Patel, Gordon A. Morris, Anna

Ebringerová, Melita Vodenicarová, Vladimír Velebny´, Alvaro Ortega, Jose Garcia de la Torre, Stephen E. Harding. Global conformation analysis of irradiated xyloglucans, Carbohydrate Polymers, 74, 845-851 (2008).

Wang Q., Huang X., Nakamura A., Burchard W., & Hallett F. R. Molecular characterization of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods, Carbohydrate Research, 340(17), 2637-2644 (2005).

Huihuang Ding. Doctoral thesis Dietary Fibres from Flaxseed Kernel: Structural and Conformational Characterization, and Structure-Function Relationship, The University of Guelph, Ontario, Canada (2015).

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