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Synthesis and characterization of curcumin-phosphatidylcholine complex via solution enhanced dispersion by supercritical CO2

Phan Minh Vuong, Nguyen Minh Ty, Pham Cao Thanh Tung, Phan Thanh Thao


In order to enhance the bioavailability of poorly water-soluble curcumin, solution enhanced dispersion by supercritical carbon dioxide (CO2) (SEDS) was employed to prepare curcumin-phosphatidyl choline complex. The reaction parameters were varied and investigated. The typical parameters were determined as follow:  P = 200 bar, T = 60 °C, gas flow rate 10 mL.min-1, molar ratio of phosphatidylcholine:curcumin 2:1, ratio of dichloromethane/scCO2 15 % (w/w). The characteristics of complexation product were measured such as the water solubility, natural stability, UV-Vis, FTIR and DSC. The FE-SEM image shows that curcumin-phosphatidylcholine complex appeared in two types of spherical shape with the big particle size of  complex approximately 0.9 µm and nano curcumin with particles size of approximately 100 nm. This study revealed that supercritical CO2 technologies had a great potential in fabricating complex and improving the bioavailability of poorly water-soluble drugs.

Keywords. Curcumin, water soluble curcumin, phosphatidylcholine, CO2 supercritical fluids.


Curcumin, water soluble curcumin, phosphatidylcholine, CO2 supercritical fluids


Glen R. B. Irving, Ankur Karmokar. Curcumin: The

potential for efficacy in gastrointestinal diseases, Best Practice & Research Clinical Gastroenterology, 25, 519-534 (2011).

Murali M. Y., Meena J., Subhash C.C. Curcumin nanoformulations: a future nanomedicine for cancer, Drug Discovery Today, 17(1-2), 71-80 (2012).

Takaaki Harada, Duc-Truc Pham, Stephen F. Lincoln, Tak W. Kee. The Capture and Stabilization of Curcumin Using Hydrophobically Modified Polyacrylate Aggregates and Hydrogels, J. Phys. Chem. B., 118, 9515-9523 (2014).

P. Munshi, S. Bhaduri. Supercritical CO2: a twenty-first century solvent for the chemical industry, Current Science, 97, 63-72 (2009).

Bhawana, K. B Rupesh, S. B Harpreet, V. K. Jain, J. Nidhi. Curcumin Nanoparticles: Preparation, Characterization, and Antimicrobial Study, J. Agric. Food Chem., 59, 2056-2061 (2011).

Xiaoxue Zhang, Saar a Heinonen and Erkki Levanen. Applications of supercritical carbon dioxide in materials processing and synthesis, RSC Adv., 4, 61137-61152 (2014).

M. Turk, P. Hils, B. Helfgen, M. A. Wahl. Micronization of pharmaceutical substances by the Rapid Expansion of Supercritical Solutions (RESS): a promising method to improve bioavailability of poorly soluble pharmaceutical agents, Journal of Supercritical Fluids, 22, 75-84 (2002).

Mahshid Kalani, Robiah Yunus. Application of supercritical antisolvent method in drug encapsulation: a review, International Journal of Nanomedicine, 6, 1429-1442 (2011).

Jagruti Patel, Rakesh Patel, Kapil Khambholja, Nirav Patel. An overview of phytosomes as an advanced herbal drug delivery system, Asian Journal of Pharmaceutical Sciences, 4(6), 363-371 (2009).

Parneet Kaur, Parul Sen, Dr Sandeep Arora, Arvind Sharma. Emerging Trends and Future Prospective of Phytosomes as Carrier for Enhanced Bioavailabilty of Bioactives: A Review, PhTechMed., 1(3) (2012).

Süleymanoglu E. The use of ir spectroscopy after rehydration to follow ternary lipoplex formation and design as a metal-based dna nanopharmaceuticals, Sec. Biol. Med. Sci., 61-80 (2009).

Kidd P. M. Bioavailability and Activity of Phytosome Complexes from Botanical Polyphenols: The Silymarin, Curcumin, Alt. Med. Rev., 14, 226-246 (2009).

Mohan P. R. K., Sreelakshmi G., Muraleedharan C. V., Roy J. Water soluble complexes of curcumin with cyclodextrins: Characterization by FT-Raman spectroscopy, Vibra. Spectro., 62, 77-84 (2012).

Bo T., Li M., Huai-You W., Guo-Ying Z. Study on the Supramolecular Interaction of Curcumin and β-cyclodextrin by Spectrophotometry and Its Analytical Application, J. Agric. Food Chem., 50, 1355-1361 (2002).

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