Screening of hybridoma cell lines secreting monoclonal antibodies specific for staphylococcal enterotoxin B (SEB) derived from Staphylococcus aureus

Nghiêm Ngọc Minh, Nguyễn Thị Hoài Thu, Phạm Thùy Linh, Thân Đức Dương, Vũ Thị Thu Hằng, Lê Văn Phan
Author affiliations

Authors

  • Nghiêm Ngọc Minh Viện Nghiên cứu hệ gen, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Nguyễn Thị Hoài Thu Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Phạm Thùy Linh Viện Công nghệ sinh học, Viện Hàn lâm Khoa học và Công nghệ Việt Nam
  • Thân Đức Dương Công ty Cổ phần Phát triển Công nghệ Nông thôn
  • Vũ Thị Thu Hằng Công ty Cổ phần Phát triển Công nghệ Nông thôn
  • Lê Văn Phan Học viện Nông nghiệp Việt Nam

DOI:

https://doi.org/10.15625/1811-4989/14/1/9288

Keywords:

ELISA, hybridoma line, monoclonal antibody, SEB recombinant antigen, Western blot

Abstract

Staphylococcus aureus (S. aureus) produces 11 types of toxins and more than 20 different Staphylococcal enterotoxins (SEs), including SEA to SEE, SEG to SER and SEU. Among them, enterotoxin type B (Staphylococcal enterotoxin B - SEB) is quite heat stable and causes gastrointestinal diseases in food poisoning. The symptoms of SEB intoxication begin with the onset of sudden fever, about 40oC to 41oC, chills, headache, muscle aches and dry cough. Some patients feel shortness of breath and chest pain. Although SEB is not considered lethal, high level of exposure can lead to shock and death. Therefore, a nontoxic SEB recombinant antigen was produced to immunize mice to create B lymphocytes. Myeloma cells were fused with the B lymphocytes to generate  hybridoma lines. The screening of monoclonal antibodies for the SEB antigen was determined by ELISA and Western blot tests. This study demonstrates that an SEB recombinant antigen can immunize a response against SEB in BALB/c mice. The production of monoclonal antibodies will be used to make a rapid detection strip for SEB based on immunochromatography.

Downloads

Download data is not yet available.

Downloads

Published

30-03-2016

How to Cite

Minh, N. N., Thu, N. T. H., Linh, P. T., Dương, T. Đức, Hằng, V. T. T., & Phan, L. V. (2016). Screening of hybridoma cell lines secreting monoclonal antibodies specific for staphylococcal enterotoxin B (SEB) derived from Staphylococcus aureus. Vietnam Journal of Biotechnology, 14(1), 23–28. https://doi.org/10.15625/1811-4989/14/1/9288

Issue

Section

Articles

Most read articles by the same author(s)